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[[Image:1oz9.gif|left|200px]]<br /><applet load="1oz9" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1oz9, resolution 1.894&Aring;" />
'''Crystal structure of AQ_1354, a hypothetical protein from Aquifex aeolicus'''<br />


==Overview==
==Crystal structure of AQ_1354, a hypothetical protein from Aquifex aeolicus==
The crystal structure of a hypothetical protein AQ_1354 (gi 2983779) from the hyperthermophilic bacteria Aquifex aeolicus has been determined using X-ray crystallography. As found in many structural genomics studies, this protein is not associated with any known function based on its amino-acid sequence. PSI-BLAST analysis against a non-redundant sequence database gave 68 similar sequences referred to as 'conserved hypothetical proteins' from the uncharacterized protein family UPF0054 (accession No. PF02310). Crystallographic analysis revealed that the overall fold of this protein consists of one central alpha-helix surrounded by a four-stranded beta-sheet and four other alpha-helices. Structure-based homology analysis with DALI revealed that the structure has a moderate to good resemblance to metal-dependent proteinases such as collagenases and gelatinases, thus suggesting its possible molecular function. However, experimental tests for collagenase and gelatinase-type function show no detectable activity under standard assay conditions. Therefore, we suggest either that the members of the UPF0054 family have a similar fold but different biochemical functions to those of collagenases and gelatinases or that they have a similar function but perform it under different conditions.
<StructureSection load='1oz9' size='340' side='right'caption='[[1oz9]], [[Resolution|resolution]] 1.89&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1oz9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OZ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OZ9 FirstGlance]. <br>
1OZ9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OZ9 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.894&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oz9 OCA], [https://pdbe.org/1oz9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oz9 RCSB], [https://www.ebi.ac.uk/pdbsum/1oz9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oz9 ProSAT], [https://www.topsan.org/Proteins/BSGC/1oz9 TOPSAN]</span></td></tr>
==Reference==
</table>
Structure of the hypothetical protein AQ_1354 from Aquifex aeolicus., Oganesyan V, Busso D, Brandsen J, Chen S, Jancarik J, Kim R, Kim SH, Acta Crystallogr D Biol Crystallogr. 2003 Jul;59(Pt 7):1219-23. Epub 2003, Jun 27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12832766 12832766]
== Function ==
[https://www.uniprot.org/uniprot/YBEY_AQUAE YBEY_AQUAE] Single strand-specific metallo-endoribonuclease involved in late-stage 70S ribosome quality control and in maturation of the 3' terminus of the 16S rRNA.[HAMAP-Rule:MF_00009]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oz/1oz9_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oz9 ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Aquifex aeolicus]]
[[Category: Aquifex aeolicus]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: BSGC, Berkeley Structural Genomics Center.]]
[[Category: Brandsen J]]
[[Category: Brandsen, J.]]
[[Category: Busso D]]
[[Category: Busso, D.]]
[[Category: Chen S]]
[[Category: Chen, S.]]
[[Category: Jancarik J]]
[[Category: Jancarik, J.]]
[[Category: Kim R]]
[[Category: Kim, R.]]
[[Category: Kim SH]]
[[Category: Kim, S H.]]
[[Category: Oganesyan V]]
[[Category: Oganesyan, V.]]
[[Category: berkeley structural genomics center]]
[[Category: bsgc structure funded by nih]]
[[Category: matrix metalloproteinase type fold]]
[[Category: protein structure initiative]]
[[Category: psi]]
[[Category: structural genomics]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:23:19 2008''

Latest revision as of 11:04, 14 February 2024

Crystal structure of AQ_1354, a hypothetical protein from Aquifex aeolicusCrystal structure of AQ_1354, a hypothetical protein from Aquifex aeolicus

Structural highlights

1oz9 is a 1 chain structure with sequence from Aquifex aeolicus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.894Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT, TOPSAN

Function

YBEY_AQUAE Single strand-specific metallo-endoribonuclease involved in late-stage 70S ribosome quality control and in maturation of the 3' terminus of the 16S rRNA.[HAMAP-Rule:MF_00009]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1oz9, resolution 1.89Å

Drag the structure with the mouse to rotate

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