1oty: Difference between revisions

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[[Image:1oty.jpg|left|200px]]


<!--
==Native PNP +ALLO==
The line below this paragraph, containing "STRUCTURE_1oty", creates the "Structure Box" on the page.
<StructureSection load='1oty' size='340' side='right'caption='[[1oty]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1oty]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OTY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OTY FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
-->
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6MP:6-METHYLPURINE'>6MP</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
{{STRUCTURE_1oty| PDB=1oty |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oty FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oty OCA], [https://pdbe.org/1oty PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oty RCSB], [https://www.ebi.ac.uk/pdbsum/1oty PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oty ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DEOD_ECOLI DEOD_ECOLI] Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules.[HAMAP-Rule:MF_01627]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ot/1oty_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oty ConSurf].
<div style="clear:both"></div>


'''Native PNP +ALLO'''
==See Also==
 
*[[Purine nucleoside phosphorylase 3D structures|Purine nucleoside phosphorylase 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
Activation of prodrugs by Escherichia coli purine nucleoside phosphorylase (PNP) provides a method for selectively killing tumor cells expressing a transfected PNP gene. This gene therapy approach requires matching a prodrug and a known enzymatic activity present only in tumor cells. The specificity of the method relies on avoiding prodrug cleavage by enzymes already present in the host cells or the intestinal flora. Using crystallographic and computer modeling methods as guides, we have redesigned E. coli PNP to cleave new prodrug substrates more efficiently than does the wild-type enzyme. In particular, the M64V PNP mutant cleaves 9-(6-deoxy-alpha-L-talofuranosyl)-6-methylpurine with a kcat/Km over 100 times greater than for native E. coli PNP. In a xenograft tumor experiment, this compound caused regression of tumors expressing the M64V PNP gene.
 
==About this Structure==
1OTY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OTY OCA].
 
==Reference==
Designer gene therapy using an Escherichia coli purine nucleoside phosphorylase/prodrug system., Bennett EM, Anand R, Allan PW, Hassan AE, Hong JS, Levasseur DN, McPherson DT, Parker WB, Secrist JA 3rd, Sorscher EJ, Townes TM, Waud WR, Ealick SE, Chem Biol. 2003 Dec;10(12):1173-81. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14700625 14700625]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Purine-nucleoside phosphorylase]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Allan PW]]
[[Category: Allan, P W.]]
[[Category: Anand R]]
[[Category: Anand, R.]]
[[Category: Bennett EM]]
[[Category: Bennett, E M.]]
[[Category: Ealick SE]]
[[Category: Ealick, S E.]]
[[Category: Hassan AE]]
[[Category: Hassan, A E.]]
[[Category: McPherson DT]]
[[Category: McPherson, D T.]]
[[Category: Parker WB]]
[[Category: Parker, W B.]]
[[Category: Secrist JA]]
[[Category: Secrist, J A.]]
[[Category: Sorscher EJ]]
[[Category: Sorscher, E J.]]
[[Category: Glycosyltransferase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 04:16:47 2008''

Latest revision as of 11:03, 14 February 2024

Native PNP +ALLONative PNP +ALLO

Structural highlights

1oty is a 3 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.5Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

DEOD_ECOLI Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules.[HAMAP-Rule:MF_01627]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1oty, resolution 2.50Å

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