1opy: Difference between revisions

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[[Image:1opy.jpg|left|200px]]<br /><applet load="1opy" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1opy, resolution 1.9&Aring;" />
'''KSI'''<br />


==Overview==
==KSI==
The three-dimensional structure of the enzyme 3-oxo-delta5-steroid isomerase (E.C. 5.3.3.1), a 28-kilodalton symmetrical dimer, was solved by multidimensional heteronuclear magnetic resonance spectroscopy. The two independently folded monomers pack together by means of extensive hydrophobic and electrostatic interactions. Each monomer comprises three alpha helices and a six-strand mixed beta-pleated sheet arranged to form a deep hydrophobic cavity. Catalytically important residues Tyr14 (general acid) and Asp38 (general base) are located near the bottom of the cavity and positioned as expected from mechanistic hypotheses. An unexpected acid group (Asp99) is also located in the active site adjacent to Tyr14, and kinetic and binding studies of the Asp99 to Ala mutant demonstrate that Asp99 contributes to catalysis by stabilizing the intermediate.
<StructureSection load='1opy' size='340' side='right'caption='[[1opy]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1opy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OPY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OPY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1opy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1opy OCA], [https://pdbe.org/1opy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1opy RCSB], [https://www.ebi.ac.uk/pdbsum/1opy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1opy ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SDIS_PSEPU SDIS_PSEPU]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/op/1opy_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1opy ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1OPY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Active as [http://en.wikipedia.org/wiki/Steroid_Delta-isomerase Steroid Delta-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1] Known structural/functional Site: <scene name='pdbsite=A:Active+Site'>A</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OPY OCA].
*[[Ketosteroid Isomerase|Ketosteroid Isomerase]]
 
__TOC__
==Reference==
</StructureSection>
Solution structure of 3-oxo-delta5-steroid isomerase., Wu ZR, Ebrahimian S, Zawrotny ME, Thornburg LD, Perez-Alvarado GC, Brothers P, Pollack RM, Summers MF, Science. 1997 Apr 18;276(5311):415-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9103200 9103200]
[[Category: Large Structures]]
[[Category: Pseudomonas putida]]
[[Category: Pseudomonas putida]]
[[Category: Single protein]]
[[Category: Cha S-S]]
[[Category: Steroid Delta-isomerase]]
[[Category: Cho H-S]]
[[Category: Cha, S S.]]
[[Category: Cho M-J]]
[[Category: Cho, H S.]]
[[Category: Choi K-Y]]
[[Category: Cho, M J.]]
[[Category: Ha N-C]]
[[Category: Choi, K Y.]]
[[Category: Kim J-S]]
[[Category: Ha, N C.]]
[[Category: Kim S-W]]
[[Category: Kim, J S.]]
[[Category: Oh B-H]]
[[Category: Kim, S W.]]
[[Category: Oh, B H.]]
[[Category: high resolution]]
[[Category: isomerase]]
[[Category: ksi]]
[[Category: low-barrier hydrogen bond]]
[[Category: pseudomonas putida biotype b]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:20:18 2008''

Latest revision as of 11:02, 14 February 2024

KSIKSI

Structural highlights

1opy is a 1 chain structure with sequence from Pseudomonas putida. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.9Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SDIS_PSEPU

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1opy, resolution 1.90Å

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