1oib: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
New page: left|200px<br /><applet load="1oib" size="450" color="white" frame="true" align="right" spinBox="true" caption="1oib, resolution 2.4Å" /> '''PHOSPHATE-BINDING PRO...
 
No edit summary
 
(15 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1oib.gif|left|200px]]<br /><applet load="1oib" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1oib, resolution 2.4&Aring;" />
'''PHOSPHATE-BINDING PROTEIN MUTANT T141D'''<br />


==Overview==
==PHOSPHATE-BINDING PROTEIN MUTANT T141D==
Electrostatic interactions are among the key forces determining the, structure and function of proteins. These are exemplified in the liganded, form of the receptor, a phosphate binding protein from Escherichia coli., The phosphate, completely dehydrated and buried in the receptor, is bound, by 12 hydrogen bonds as well as a salt link with Arg 135. We have, modulated the ionic attraction while preserving the hydrogen bonds by, mutating Asp 137, also salt linked to Arg 135, to Asn, Gly or Thr., High-resolution crystallographic analysis revealed that Gly and Thr (but, not Asn) mutant proteins have incorporated a more electronegative Cl- in, place of the Asp carboxylate. That no dramatic effect on phosphate, affinity was produced by these ionic perturbations indicates a major role, for hydrogen bonds and other local dipoles in the binding and charge, stabilization of ionic ligands.
<StructureSection load='1oib' size='340' side='right'caption='[[1oib]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1oib]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OIB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OIB FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oib FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oib OCA], [https://pdbe.org/1oib PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oib RCSB], [https://www.ebi.ac.uk/pdbsum/1oib PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oib ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PSTS_ECOLI PSTS_ECOLI] Part of the ABC transporter complex PstSACB involved in phosphate import.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oi/1oib_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oib ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1OIB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OIB OCA].
*[[Phosphate-binding protein|Phosphate-binding protein]]
 
__TOC__
==Reference==
</StructureSection>
Modulation of a salt link does not affect binding of phosphate to its specific active transport receptor., Yao N, Ledvina PS, Choudhary A, Quiocho FA, Biochemistry. 1996 Feb 20;35(7):2079-85. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8652549 8652549]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Choudhary, A.]]
[[Category: Choudhary A]]
[[Category: Ledvina, P.S.]]
[[Category: Ledvina PS]]
[[Category: Quiocho, F.A.]]
[[Category: Quiocho FA]]
[[Category: Yao, N.]]
[[Category: Yao N]]
[[Category: phosphate transport]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:58:06 2007''

Latest revision as of 11:01, 14 February 2024

PHOSPHATE-BINDING PROTEIN MUTANT T141DPHOSPHATE-BINDING PROTEIN MUTANT T141D

Structural highlights

1oib is a 2 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.4Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PSTS_ECOLI Part of the ABC transporter complex PstSACB involved in phosphate import.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1oib, resolution 2.40Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=1oib&oldid=4052796"