1nyh: Difference between revisions

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New page: left|200px<br /><applet load="1nyh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nyh, resolution 3.10Å" /> '''Crystal Structure of...
 
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[[Image:1nyh.jpg|left|200px]]<br /><applet load="1nyh" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1nyh, resolution 3.10&Aring;" />
'''Crystal Structure of the Coiled-coil Dimerization Motif of Sir4'''<br />


==Overview==
==Crystal Structure of the Coiled-coil Dimerization Motif of Sir4==
The yeast silent information regulators Sir2, Sir3, and Sir4 physically, interact with one another to establish a transcriptionally silent state by, forming repressive chromatin structures. The Sir4 protein contains binding, sites for both Sir2 and Sir3, and these protein-protein interactions are, required for gene silencing. Here, we report the X-ray structure of the, coiled-coil dimerization motif within the C-terminus of Sir4 and show that, it forms a stable 1:1 complex with a dimeric fragment of Sir3 (residues, 464-978). We have identified a cluster of residues on the surface of the, Sir4 coiled coil required for specific interactions with Sir3. The histone, deacetylase Sir2 can also bind to this complex, forming a ternary complex, with the truncated Sir3 and Sir4 proteins. The dual interactions of Sir4, with Sir3 and Sir2 suggest a physical basis for recruiting Sir3 to, chromatin by virtue of its interactions with Sir4 and with deacetylated, histones in chromatin.
<StructureSection load='1nyh' size='340' side='right'caption='[[1nyh]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1nyh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NYH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NYH FirstGlance]. <br>
1NYH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NYH OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nyh OCA], [https://pdbe.org/1nyh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nyh RCSB], [https://www.ebi.ac.uk/pdbsum/1nyh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nyh ProSAT]</span></td></tr>
==Reference==
</table>
Structure of the coiled-coil dimerization motif of Sir4 and its interaction with Sir3., Chang JF, Hall BE, Tanny JC, Moazed D, Filman D, Ellenberger T, Structure. 2003 Jun;11(6):637-49. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12791253 12791253]
== Function ==
[https://www.uniprot.org/uniprot/SIR4_YEAST SIR4_YEAST] The proteins SIR1 through SIR4 are required for transcriptional repression of the silent mating type loci, HML and HMR. The proteins SIR2 through SIR4 repress mulitple loci by modulating chromatin structure. Involves the compaction of chromatin fiber into a more condensed form.<ref>PMID:18039933</ref>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Single protein]]
[[Category: Chang JF]]
[[Category: Chang, J.F.]]
[[Category: Ellenberger T]]
[[Category: Ellenberger, T.]]
[[Category: Filman D]]
[[Category: Filman, D.]]
[[Category: Hall BE]]
[[Category: Hall, B.E.]]
[[Category: Moazed D]]
[[Category: Moazed, D.]]
[[Category: Tanny JC]]
[[Category: Tanny, J.C.]]
[[Category: coiled-coil]]
[[Category: repressor]]
[[Category: transcription regulation]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:39:39 2007''

Latest revision as of 11:00, 14 February 2024

Crystal Structure of the Coiled-coil Dimerization Motif of Sir4Crystal Structure of the Coiled-coil Dimerization Motif of Sir4

Structural highlights

1nyh is a 1 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.1Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SIR4_YEAST The proteins SIR1 through SIR4 are required for transcriptional repression of the silent mating type loci, HML and HMR. The proteins SIR2 through SIR4 repress mulitple loci by modulating chromatin structure. Involves the compaction of chromatin fiber into a more condensed form.[1]

References

  1. ↑ Bupp JM, Martin AE, Stensrud ES, Jaspersen SL. Telomere anchoring at the nuclear periphery requires the budding yeast Sad1-UNC-84 domain protein Mps3. J Cell Biol. 2007 Dec 3;179(5):845-54. Epub 2007 Nov 26. PMID:18039933 doi:http://dx.doi.org/10.1083/jcb.200706040

1nyh, resolution 3.10Å

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