1nt2: Difference between revisions

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-[[Image:1nt2.jpg|left|200px]]<br /><applet load="1nt2" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1nt2, resolution 2.90&Aring;" />
-'''CRYSTAL STRUCTURE OF FIBRILLARIN/NOP5P COMPLEX'''<br />
-==Overview==
+==CRYSTAL STRUCTURE OF FIBRILLARIN/NOP5P COMPLEX==
-Nop56p and Nop58p are two core proteins of the box C/D snoRNPs that, interact concurrently with fibrillarin and snoRNAs to function in enzyme, assembly and catalysis. Here we report the 2.9 A resolution co-crystal, structure of an archaeal homolog of Nop56p/Nop58p, Nop5p, in complex with, fibrillarin from Archaeoglobus fulgidus (AF) and the methyl donor, S-adenosyl-L-methionine. The N-terminal domain of Nop5p forms a, complementary surface to fibrillarin that serves to anchor the catalytic, subunit and to stabilize cofactor binding. A coiled coil in Nop5p mediates, dimerization of two fibrillarin-Nop5p heterodimers for optimal, interactions with bipartite box C/D RNAs. Structural analysis and, complementary biochemical data demonstrate that the conserved C-terminal, domain of Nop5p harbors RNA-binding sites. A model of box C/D snoRNP, assembly is proposed based on the presented structural and biochemical, data.
+<StructureSection load='1nt2' size='340' side='right'caption='[[1nt2]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[1nt2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_DSM_4304 Archaeoglobus fulgidus DSM 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NT2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NT2 FirstGlance]. <br>
-NT2 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus] with SAM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NT2 OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
-==Reference==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nt2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nt2 OCA], [https://pdbe.org/1nt2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nt2 RCSB], [https://www.ebi.ac.uk/pdbsum/1nt2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nt2 ProSAT]</span></td></tr>
-Structure and function of archaeal box C/D sRNP core proteins., Aittaleb M, Rashid R, Chen Q, Palmer JR, Daniels CJ, Li H, Nat Struct Biol. 2003 Apr;10(4):256-63. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12598892 12598892]
+</table>
-[[Category: Archaeoglobus fulgidus]]
+== Function ==
-[[Category: Protein complex]]
+[https://www.uniprot.org/uniprot/FLPA_ARCFU FLPA_ARCFU] Involved in pre-rRNA and tRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in rRNA and tRNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA.<ref>PMID:15286083</ref>
-[[Category: Aittaleb, M.]]
+== Evolutionary Conservation ==
-[[Category: Chen, Q.]]
+[[Image:Consurf_key_small.gif|200px|right]]
-[[Category: Daniels, C.J.]]
+Check<jmol>
-[[Category: Li, H.]]
+  <jmolCheckbox>
-[[Category: Palmer, J.R.]]
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nt/1nt2_consurf.spt"</scriptWhenChecked>
-[[Category: Rashid, R.]]
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-[[Category: SAM]]
+    <text>to colour the structure by Evolutionary Conservation</text>
-[[Category: ademet]]
+  </jmolCheckbox>
-[[Category: binding motif]]
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nt2 ConSurf].
-[[Category: coiled coil]]
+<div style="clear:both"></div>
+== References ==
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:31:56 2007''
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Archaeoglobus fulgidus DSM 4304]]
+[[Category: Large Structures]]
+[[Category: Aittaleb M]]
+[[Category: Chen Q]]
+[[Category: Daniels CJ]]
+[[Category: Li H]]
+[[Category: Palmer JR]]
+[[Category: Rashid R]]