1nsg: Difference between revisions

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OCA

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-[[Image:1nsg.gif|left|200px]]<br />
-<applet load="1nsg" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1nsg, resolution 2.2&Aring;" />
-'''THE STRUCTURE OF THE IMMUNOPHILIN-IMMUNOSUPPRESSANT FKBP12-RAPAMYCIN COMPLEX INTERACTING WITH HUMAN FRAP'''<br />
-==Overview==
+==THE STRUCTURE OF THE IMMUNOPHILIN-IMMUNOSUPPRESSANT FKBP12-RAPAMYCIN COMPLEX INTERACTING WITH HUMAN FRAP==
-The structure of the FKBP12-rapamycin-FRB ternary complex has now been, refined at 2.2 A resolution. The cell-cycle arrest agent rapamycin binds, FK506-binding protein (FKBP12) and the FKBP12-rapamycin binding (FRB), domain of FKBP12-rapamycin associated protein (FRAP) simultaneously, and, the inhibition of FRAP is responsible for rapamycin's biological activity., The conformation of rapamycin in the ternary complex is very similar to, that observed in the FKBP12-rapamycin binary complex, with an r.m.s., difference of only 0.30 A. However, a slight (9 degrees ) rotation, repositions the FRB-binding face of rapamycin in the ternary complex., There are extensive rapamycin-protein interactions and relatively few, interactions between the two protein partners FKBP12 and FRB, these, interactions mainly involving residues in the 40s and 80s loops of FKBP12, and alpha1 and alpha4 of FRB. The high-resolution refinement has revealed, the crucial role of several buried waters in the formation of the ternary, complex.
+<StructureSection load='1nsg' size='340' side='right'caption='[[1nsg]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1nsg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NSG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NSG FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=RAD:C49-METHYL+RAPAMYCIN'>RAD</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nsg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nsg OCA], [https://pdbe.org/1nsg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nsg RCSB], [https://www.ebi.ac.uk/pdbsum/1nsg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nsg ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/FKB1A_HUMAN FKB1A_HUMAN] Keeps in an inactive conformation TGFBR1, the TGF-beta type I serine/threonine kinase receptor, preventing TGF-beta receptor activation in absence of ligand. Recruites SMAD7 to ACVR1B which prevents the association of SMAD2 and SMAD3 with the activin receptor complex, thereby blocking the activin signal. May modulate the RYR1 calcium channel activity. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.<ref>PMID:9233797</ref> <ref>PMID:16720724</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ns/1nsg_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nsg ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-NSG is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with RAD as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Peptidylprolyl_isomerase Peptidylprolyl isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.2.1.8 5.2.1.8] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NSG OCA].
+*[[FKBP 3D structures|FKBP 3D structures]]
+*[[Serine/threonine protein kinase 3D structures|Serine/threonine protein kinase 3D structures]]
-==Reference==
+== References ==
-Refined structure of the FKBP12-rapamycin-FRB ternary complex at 2.2 A resolution., Liang J, Choi J, Clardy J, Acta Crystallogr D Biol Crystallogr. 1999 Apr;55(Pt 4):736-44. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10089303 10089303]
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Peptidylprolyl isomerase]]
+[[Category: Large Structures]]
-[[Category: Protein complex]]
+[[Category: Choi J]]
-[[Category: Choi, J.]]
+[[Category: Clardy J]]
-[[Category: Clardy, J.]]
+[[Category: Liang J]]
-[[Category: Liang, J.]]
-[[Category: RAD]]
-[[Category: complex (isomerase/kinase)]]
-[[Category: fkbp12]]
-[[Category: frap]]
-[[Category: rapamycin]]
-[[Category: transferase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 18:24:51 2007''