1nsf: Difference between revisions

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-[[Image:1nsf.gif|left|200px]]<br />
-<applet load="1nsf" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1nsf, resolution 1.90&Aring;" />
-'''D2 HEXAMERIZATION DOMAIN OF N-ETHYLMALEIMIDE SENSITIVE FACTOR (NSF)'''<br />
-==Overview==
+==D2 HEXAMERIZATION DOMAIN OF N-ETHYLMALEIMIDE SENSITIVE FACTOR (NSF)==
-N-ethylmaleimide-sensitive factor (NSF) is a hexameric ATPase which primes, and/or dissociates SNARE complexes involved in intracellular fusion, events. Each NSF protomer contains three domains: an N-terminal domain, required for SNARE binding and two ATPase domains, termed D1 and D2, with, D2 being required for oligomerization. We have determined the 1.9 A, crystal structure of the D2 domain of NSF complexed with ATP using, multi-wavelength anomalous dispersion phasing. D2 consists of a nucleotide, binding subdomain with a Rossmann fold and a C-terminal subdomain, which, is structurally unique among nucleotide binding proteins. There are, interactions between the ATP moiety and both the neighboring D2 protomer, and the C-terminal subdomain that may be important for ATP-dependent, oligomerization. Of particular importance are three well-ordered and, conserved lysine residues that form ionic interactions with the beta- and, gamma-phosphates, one of which likely contributes to the low hydrolytic, activity of D2.
+<StructureSection load='1nsf' size='340' side='right'caption='[[1nsf]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[1nsf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus]. The August 2006 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''AAA+ Proteases''  by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2006_8 10.2210/rcsb_pdb/mom_2006_8]. The November 2013 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''SNARE Proteins''  by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2013_11 10.2210/rcsb_pdb/mom_2013_11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NSF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NSF FirstGlance]. <br>
-NSF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus] with MG and ATP as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1NSF with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb80_1.html AAA+ Proteases]]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NSF OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-==Reference==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nsf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nsf OCA], [https://pdbe.org/1nsf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nsf RCSB], [https://www.ebi.ac.uk/pdbsum/1nsf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nsf ProSAT]</span></td></tr>
-Structure of the ATP-dependent oligomerization domain of N-ethylmaleimide sensitive factor complexed with ATP., Yu RC, Hanson PI, Jahn R, Brunger AT, Nat Struct Biol. 1998 Sep;5(9):803-11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9731775 9731775]
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/NSF_CRIGR NSF_CRIGR] Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ns/1nsf_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nsf ConSurf].
+<div style="clear:both"></div>
+__TOC__
+</StructureSection>
 [[Category: AAA+ Proteases]]
 [[Category: Cricetulus griseus]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Brunger, A.T.]]
+[[Category: RCSB PDB Molecule of the Month]]
-[[Category: Hanson, P.I.]]
+[[Category: SNARE Proteins]]
-[[Category: Jahn, R.]]
+[[Category: Brunger AT]]
-[[Category: Yu, R.C.]]
+[[Category: Hanson PI]]
-[[Category: ATP]]
+[[Category: Jahn R]]
-[[Category: MG]]
+[[Category: Yu RC]]
-[[Category: atp-binding]]
-[[Category: endoplasmic reticulum]]
-[[Category: golgi stack]]
-[[Category: protein transport]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:04:21 2007''