1nof: Difference between revisions

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New page: left|200px<br /> <applet load="1nof" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nof, resolution 1.42Å" /> '''THE FIRST CRYSTALLO...
 
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[[Image:1nof.gif|left|200px]]<br />
<applet load="1nof" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1nof, resolution 1.42&Aring;" />
'''THE FIRST CRYSTALLOGRAPHIC STRUCTURE OF A XYLANASE FROM GLYCOSYL HYDROLASE FAMILY 5: IMPLICATIONS FOR CATALYSIS'''<br />


==Overview==
==THE FIRST CRYSTALLOGRAPHIC STRUCTURE OF A XYLANASE FROM GLYCOSYL HYDROLASE FAMILY 5: IMPLICATIONS FOR CATALYSIS==
The room-temperature structure of xylanase (EC 3.2.1.8) from the bacterial, plant pathogen Erwinia chrysanthemi expressed in Escherichia coli, a 45, kDa, 413-amino acid protein belonging to glycoside hydrolase family 5, has, been determined by multiple isomorphous replacement and refined to a, resolution of 1.42 A. This represents the first structure of a xylanase, not belonging to either glycoside hydrolase family 10 or family 11. The, enzyme is composed of two domains similar to most family 10 xylanases and, the alpha-amylases. The catalytic domain (residues 46-315) has a, (beta/alpha)(8)-barrel motif with a binding cleft along the C-terminal, side of the beta-barrel. The catalytic residues, Glu165 and Glu253, determined by correspondence to other family 5 and family 10 glycoside, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?12859186 (full description)]]
<StructureSection load='1nof' size='340' side='right'caption='[[1nof]], [[Resolution|resolution]] 1.42&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1nof]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dickeya_chrysanthemi Dickeya chrysanthemi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NOF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NOF FirstGlance]. <br>
1NOF is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi]] with ACT as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NOF OCA]].
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.42&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nof FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nof OCA], [https://pdbe.org/1nof PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nof RCSB], [https://www.ebi.ac.uk/pdbsum/1nof PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nof ProSAT]</span></td></tr>
First crystallographic structure of a xylanase from glycoside hydrolase family 5: implications for catalysis., Larson SB, Day J, Barba de la Rosa AP, Keen NT, McPherson A, Biochemistry. 2003 Jul 22;42(28):8411-22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12859186 12859186]
</table>
[[Category: Erwinia chrysanthemi]]
== Function ==
[[Category: Single protein]]
[https://www.uniprot.org/uniprot/Q46961_DICCH Q46961_DICCH]
[[Category: Day, J.]]
== Evolutionary Conservation ==
[[Category: Keen, N.T.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Larson, S.B.]]
Check<jmol>
[[Category: McPherson, A.]]
  <jmolCheckbox>
[[Category: Rosa, A.P.Barba.De.La.]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/no/1nof_consurf.spt"</scriptWhenChecked>
[[Category: ACT]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: carbohydrate-binding module]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: catalytic domain]]
  </jmolCheckbox>
[[Category: glycohydrolase family 5]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nof ConSurf].
[[Category: xylanase]]
<div style="clear:both"></div>
 
__TOC__
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 21:50:46 2007''
</StructureSection>
[[Category: Dickeya chrysanthemi]]
[[Category: Large Structures]]
[[Category: Barba De La Rosa AP]]
[[Category: Day J]]
[[Category: Keen NT]]
[[Category: Larson SB]]
[[Category: McPherson A]]

Latest revision as of 10:58, 14 February 2024

THE FIRST CRYSTALLOGRAPHIC STRUCTURE OF A XYLANASE FROM GLYCOSYL HYDROLASE FAMILY 5: IMPLICATIONS FOR CATALYSISTHE FIRST CRYSTALLOGRAPHIC STRUCTURE OF A XYLANASE FROM GLYCOSYL HYDROLASE FAMILY 5: IMPLICATIONS FOR CATALYSIS

Structural highlights

1nof is a 1 chain structure with sequence from Dickeya chrysanthemi. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.42Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q46961_DICCH

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1nof, resolution 1.42Å

Drag the structure with the mouse to rotate

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