1nof: Difference between revisions

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-[[Image:1nof.gif|left|200px]]<br />
-<applet load="1nof" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1nof, resolution 1.42&Aring;" />
-'''THE FIRST CRYSTALLOGRAPHIC STRUCTURE OF A XYLANASE FROM GLYCOSYL HYDROLASE FAMILY 5: IMPLICATIONS FOR CATALYSIS'''<br />
-==Overview==
+==THE FIRST CRYSTALLOGRAPHIC STRUCTURE OF A XYLANASE FROM GLYCOSYL HYDROLASE FAMILY 5: IMPLICATIONS FOR CATALYSIS==
-The room-temperature structure of xylanase (EC 3.2.1.8) from the bacterial, plant pathogen Erwinia chrysanthemi expressed in Escherichia coli, a 45, kDa, 413-amino acid protein belonging to glycoside hydrolase family 5, has, been determined by multiple isomorphous replacement and refined to a, resolution of 1.42 A. This represents the first structure of a xylanase, not belonging to either glycoside hydrolase family 10 or family 11. The, enzyme is composed of two domains similar to most family 10 xylanases and, the alpha-amylases. The catalytic domain (residues 46-315) has a, (beta/alpha)(8)-barrel motif with a binding cleft along the C-terminal, side of the beta-barrel. The catalytic residues, Glu165 and Glu253, determined by correspondence to other family 5 and family 10 glycoside, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?12859186 (full description)]]
+<StructureSection load='1nof' size='340' side='right'caption='[[1nof]], [[Resolution|resolution]] 1.42&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[1nof]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dickeya_chrysanthemi Dickeya chrysanthemi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NOF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NOF FirstGlance]. <br>
-NOF is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi]] with ACT as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8]]. Structure known Active Sites: ACI and NUC. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NOF OCA]].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.42&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>
-==Reference==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nof FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nof OCA], [https://pdbe.org/1nof PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nof RCSB], [https://www.ebi.ac.uk/pdbsum/1nof PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nof ProSAT]</span></td></tr>
-First crystallographic structure of a xylanase from glycoside hydrolase family 5: implications for catalysis., Larson SB, Day J, Barba de la Rosa AP, Keen NT, McPherson A, Biochemistry. 2003 Jul 22;42(28):8411-22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12859186 12859186]
+</table>
-[[Category: Endo-1,4-beta-xylanase]]
+== Function ==
-[[Category: Erwinia chrysanthemi]]
+[https://www.uniprot.org/uniprot/Q46961_DICCH Q46961_DICCH]
-[[Category: Single protein]]
+== Evolutionary Conservation ==
-[[Category: Day, J.]]
+[[Image:Consurf_key_small.gif|200px|right]]
-[[Category: Keen, N.T.]]
+Check<jmol>
-[[Category: Larson, S.B.]]
+  <jmolCheckbox>
-[[Category: McPherson, A.]]
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/no/1nof_consurf.spt"</scriptWhenChecked>
-[[Category: Rosa, A.P.Barba.De.La.]]
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-[[Category: ACT]]
+    <text>to colour the structure by Evolutionary Conservation</text>
-[[Category: carbohydrate-binding module]]
+  </jmolCheckbox>
-[[Category: catalytic domain]]
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nof ConSurf].
-[[Category: glycohydrolase family 5]]
+<div style="clear:both"></div>
-[[Category: xylanase]]
+__TOC__
+</StructureSection>
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:19:08 2007''
+[[Category: Dickeya chrysanthemi]]
+[[Category: Large Structures]]
+[[Category: Barba De La Rosa AP]]
+[[Category: Day J]]
+[[Category: Keen NT]]
+[[Category: Larson SB]]
+[[Category: McPherson A]]