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| [[Image:1nbs.gif|left|200px]]<br /><applet load="1nbs" size="350" color="white" frame="true" align="right" spinBox="true"
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| caption="1nbs, resolution 3.15Å" />
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| '''Crystal structure of the specificity domain of Ribonuclease P RNA'''<br />
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| ==Overview== | | ==Crystal structure of the specificity domain of Ribonuclease P RNA== |
| RNase P is the only endonuclease responsible for processing the 5' end of transfer RNA by cleaving a precursor and leading to tRNA maturation. It contains an RNA component and a protein component and has been identified in all organisms. It was one of the first catalytic RNAs identified and the first that acts as a multiple-turnover enzyme in vivo. RNase P and the ribosome are so far the only two ribozymes known to be conserved in all kingdoms of life. The RNA component of bacterial RNase P can catalyse pre-tRNA cleavage in the absence of the RNase P protein in vitro and consists of two domains: a specificity domain and a catalytic domain. Here we report a 3.15-A resolution crystal structure of the 154-nucleotide specificity domain of Bacillus subtilis RNase P. The structure reveals the architecture of this domain, the interactions that maintain the overall fold of the molecule, a large non-helical but well-structured module that is conserved in all RNase P RNA, and the regions that are involved in interactions with the substrate.
| | <StructureSection load='1nbs' size='340' side='right'caption='[[1nbs]], [[Resolution|resolution]] 3.15Å' scene=''> |
| | == Structural highlights == |
| | <table><tr><td colspan='2'>[[1nbs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NBS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NBS FirstGlance]. <br> |
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.15Å</td></tr> |
| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PB:LEAD+(II)+ION'>PB</scene></td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nbs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nbs OCA], [https://pdbe.org/1nbs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nbs RCSB], [https://www.ebi.ac.uk/pdbsum/1nbs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nbs ProSAT]</span></td></tr> |
| | </table> |
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| ==About this Structure== | | ==See Also== |
| 1NBS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=PB:'>PB</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NBS OCA].
| | *[[Ribonuclease 3D structures|Ribonuclease 3D structures]] |
| | | __TOC__ |
| ==Reference==
| | </StructureSection> |
| Crystal structure of the specificity domain of ribonuclease P., Krasilnikov AS, Yang X, Pan T, Mondragon A, Nature. 2003 Feb 13;421(6924):760-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12610630 12610630]
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| [[Category: Bacillus subtilis]] | | [[Category: Bacillus subtilis]] |
| [[Category: Single protein]] | | [[Category: Large Structures]] |
| [[Category: Krasilnikov, A S.]] | | [[Category: Krasilnikov AS]] |
| [[Category: Mondragon, A.]] | | [[Category: Mondragon A]] |
| [[Category: Pan, T.]] | | [[Category: Pan T]] |
| [[Category: Yang, X.]] | | [[Category: Yang X]] |
| [[Category: MG]]
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| [[Category: PB]]
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| [[Category: p rna]]
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| [[Category: ribonuclease p rna]]
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| [[Category: s-domain]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:04:23 2008''
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