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[[Image:1nat.gif|left|200px]]


{{Structure
==CRYSTAL STRUCTURE OF SPOOF FROM BACILLUS SUBTILIS==
|PDB= 1nat |SIZE=350|CAPTION= <scene name='initialview01'>1nat</scene>, resolution 2.45&Aring;
<StructureSection load='1nat' size='340' side='right'caption='[[1nat]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND=  
<table><tr><td colspan='2'>[[1nat]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NAT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NAT FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45&#8491;</td></tr>
|GENE= SPO0F ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nat OCA], [https://pdbe.org/1nat PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nat RCSB], [https://www.ebi.ac.uk/pdbsum/1nat PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nat ProSAT]</span></td></tr>
}}
</table>
== Function ==
[https://www.uniprot.org/uniprot/SP0F_BACSU SP0F_BACSU] Key element in the phosphorelay regulating sporulation initiation. Phosphorylation of spo0B during sporulation initiation.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/na/1nat_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nat ConSurf].
<div style="clear:both"></div>


'''CRYSTAL STRUCTURE OF SPOOF FROM BACILLUS SUBTILIS'''
==See Also==
 
*[[Phosphotransferase 3D structures|Phosphotransferase 3D structures]]
 
*[[Response regulator 3D structure|Response regulator 3D structure]]
==Overview==
__TOC__
Spo0F is a secondary messenger in the "two-component" system controlling the sporulation of Bacillus subtilis. Spo0F, like the chemotaxis protein CheY, is a single-domain protein homologous to the N-terminal activator domain of the response regulators. We recently reported the crystal structure of a phosphatase-resistant mutant Y13S of Spo0F with Ca2+ bound in the active site. The crystal structure of wild-type Spo0F in the absence of a metal ion is presented here. A comparison of the two structures reveals that the cation induces significant changes in the active site. In the present wild-type structure, the carboxylate of Asp11 points away from the center of the active site, whereas when coordinated to the Ca2+, as in the earlier structure, it points toward the active site. In addition, Asp54, the site of phosphorylation, is blocked by a salt bridge interaction of an Arg side chain from a neighboring molecule. From fluorescence quenching studies with Spo0F Y13W, we found that only the amino acid Arg binds to Spo0F in a saturable manner (Kd = 15 mM). This observation suggests that a small molecule with a shape complementary to the active site and having a guanidinium group might inhibit phosphotransfer between response regulators and their cognate histidine kinases.
</StructureSection>
 
==About this Structure==
1NAT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NAT OCA].
 
==Reference==
A response regulatory protein with the site of phosphorylation blocked by an arginine interaction: crystal structure of Spo0F from Bacillus subtilis., Madhusudan M, Zapf J, Hoch JA, Whiteley JM, Xuong NH, Varughese KI, Biochemistry. 1997 Oct 21;36(42):12739-45. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9335530 9335530]
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Hoch, J A.]]
[[Category: Hoch JA]]
[[Category: Madhusudan]]
[[Category: Madhusudan]]
[[Category: Varughese, K I.]]
[[Category: Varughese KI]]
[[Category: Whiteley, J M.]]
[[Category: Whiteley JM]]
[[Category: Xuong, N H.]]
[[Category: Xuong NH]]
[[Category: Zapf, J.]]
[[Category: Zapf J]]
[[Category: aspartate pocket]]
[[Category: regulatory protein]]
[[Category: sporulation response regulator]]
[[Category: two component system]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:54:21 2008''

Latest revision as of 10:54, 14 February 2024

CRYSTAL STRUCTURE OF SPOOF FROM BACILLUS SUBTILISCRYSTAL STRUCTURE OF SPOOF FROM BACILLUS SUBTILIS

Structural highlights

1nat is a 1 chain structure with sequence from Bacillus subtilis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.45Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SP0F_BACSU Key element in the phosphorelay regulating sporulation initiation. Phosphorylation of spo0B during sporulation initiation.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1nat, resolution 2.45Å

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