1n7a: Difference between revisions

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New page: left|200px<br /><applet load="1n7a" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n7a, resolution 1.20Å" /> '''RIP-Radiation-damage...
 
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[[Image:1n7a.gif|left|200px]]<br /><applet load="1n7a" size="450" color="white" frame="true" align="right" spinBox="true"
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'''RIP-Radiation-damage Induced Phasing'''<br />


==Overview==
==RIP-Radiation-damage Induced Phasing==
The use of third generation synchrotron sources has led to renewed concern, about the effect of ionizing radiation on crystalline biological samples., In general, the problem is seen as one to be avoided. However, in this, paper, it is shown that, far from being a hindrance to successful, structure determination, radiation damage provides an opportunity for, phasing macromolecular structures. This is successfully demonstrated for, both a protein and an oligonucleotide, by way of which complete models, were built automatically. The possibility that, through the exploitation, of radiation damage, the phase problem could become less of a barrier to, macromolecular crystal structure determination is discussed.
<StructureSection load='1n7a' size='340' side='right'caption='[[1n7a]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1n7a]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N7A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N7A FirstGlance]. <br>
1N7A is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with K and SPM as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1N7A OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGM:8-BROMO-2-DEOXYGUANOSINE-5-MONOPHOSPHATE'>BGM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=SPM:SPERMINE'>SPM</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n7a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n7a OCA], [https://pdbe.org/1n7a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n7a RCSB], [https://www.ebi.ac.uk/pdbsum/1n7a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n7a ProSAT]</span></td></tr>
Specific radiation damage can be used to solve macromolecular crystal structures., Ravelli RB, Leiros HK, Pan B, Caffrey M, McSweeney S, Structure. 2003 Feb;11(2):217-24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12575941 12575941]
</table>
[[Category: Protein complex]]
__TOC__
[[Category: Caffrey, M.]]
</StructureSection>
[[Category: Leiros, H.K.S.]]
[[Category: Large Structures]]
[[Category: McSweeney, S.]]
[[Category: Caffrey M]]
[[Category: Pan, B.]]
[[Category: Leiros H-KS]]
[[Category: Ravelli, R.B.G.]]
[[Category: McSweeney S]]
[[Category: K]]
[[Category: Pan B]]
[[Category: SPM]]
[[Category: Ravelli RBG]]
[[Category: dna]]
[[Category: rna]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 22:40:24 2007''

Latest revision as of 10:53, 14 February 2024

RIP-Radiation-damage Induced PhasingRIP-Radiation-damage Induced Phasing

Structural highlights

1n7a is a 8 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.2Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

1n7a, resolution 1.20Å

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