1n6j: Difference between revisions

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New page: left|200px<br /> <applet load="1n6j" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n6j, resolution 2.20Å" /> '''Structural basis of...
 
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[[Image:1n6j.gif|left|200px]]<br />
<applet load="1n6j" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1n6j, resolution 2.20&Aring;" />
'''Structural basis of sequence-specific recruitment of histone deacetylases by Myocyte Enhancer Factor-2'''<br />


==Overview==
==Structural basis of sequence-specific recruitment of histone deacetylases by Myocyte Enhancer Factor-2==
The myocyte enhancer factor-2 (MEF2) family of transcription factors has, important roles in the development and function of T cells, neuronal cells, and muscle cells. MEF2 is capable of repressing or activating, transcription by association with a variety of co-repressors or, co-activators in a calcium-dependent manner. Transcriptional repression by, MEF2 has attracted particular attention because of its potential role in, hypertrophic responses of cardiomyocytes. Several MEF2 co-repressors, such, as Cabin1/Cain and class II histone deacetylases (HDACs), have been, identified. However, the molecular mechanism of their recruitment to, specific promoters by MEF2 remains largely unknown. Here we report a, crystal structure of the MADS-box/MEF2S domain of human MEF2B bound to a, motif of the transcriptional co-repressor Cabin1 and DNA at 2.2 A, resolution. The crystal structure reveals a stably folded MEF2S domain on, the surface of the MADS box. Cabin1 adopts an amphipathic alpha-helix to, bind a hydrophobic groove on the MEF2S domain, forming a triple-helical, interaction. Our studies of the ternary Cabin1/MEF2/DNA complex show a, general mechanism by which MEF2 recruits transcriptional co-repressor, Cabin1 and class II HDACs to specific DNA sites.
<StructureSection load='1n6j' size='340' side='right'caption='[[1n6j]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1n6j]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N6J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N6J FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n6j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n6j OCA], [https://pdbe.org/1n6j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n6j RCSB], [https://www.ebi.ac.uk/pdbsum/1n6j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n6j ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MEF2B_HUMAN MEF2B_HUMAN] Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific genes. Activates transcription via this element. May be involved in muscle-specific and/or growth factor-related transcription.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n6/1n6j_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n6j ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1N6J is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1N6J OCA].
*[[Myocyte enhancer factor 2|Myocyte enhancer factor 2]]
 
__TOC__
==Reference==
</StructureSection>
Sequence-specific recruitment of transcriptional co-repressor Cabin1 by myocyte enhancer factor-2., Han A, Pan F, Stroud JC, Youn HD, Liu JO, Chen L, Nature. 2003 Apr 17;422(6933):730-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12700764 12700764]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Protein complex]]
[[Category: Large Structures]]
[[Category: Chen, L.]]
[[Category: Chen L]]
[[Category: Han, A.]]
[[Category: Han A]]
[[Category: Liu, J.O.]]
[[Category: Liu JO]]
[[Category: Pan, F.]]
[[Category: Pan F]]
[[Category: Stroud, J.C.]]
[[Category: Stroud JC]]
[[Category: Youn, H.D.]]
[[Category: Youn HD]]
[[Category: histone deacetylases]]
[[Category: mads-box]]
[[Category: protein-dna complex]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 18:18:12 2007''

Latest revision as of 10:53, 14 February 2024

Structural basis of sequence-specific recruitment of histone deacetylases by Myocyte Enhancer Factor-2Structural basis of sequence-specific recruitment of histone deacetylases by Myocyte Enhancer Factor-2

Structural highlights

1n6j is a 5 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.2Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MEF2B_HUMAN Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific genes. Activates transcription via this element. May be involved in muscle-specific and/or growth factor-related transcription.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1n6j, resolution 2.20Å

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