1n2f: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1n2f.gif|left|200px]]<br /><applet load="1n2f" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="1n2f, resolution 2.01&Aring;" />
-'''CRYSTAL STRUCTURE OF P. AERUGINOSA OHR'''<br />
-==Overview==
+==CRYSTAL STRUCTURE OF P. AERUGINOSA OHR==
-Bacteria have developed complex strategies to detoxify and repair damage caused by reactive oxygen species. These compounds, produced during bacterial aerobic respiration as well as by the host immune system cells as a defense mechanism against the pathogenic microorganisms, have the ability to damage nucleic acids, proteins and phospholipid membranes. Here we describe the crystal structure of Pseudomonas aeruginosa Ohr, a member of a recently discovered family of organic hydroperoxide resistance proteins. Ohr is a tightly folded homodimer, with a novel alpha/beta fold, and contains two active sites located at the monomer interface on opposite sides of the molecule. Using in vitro assays, we demonstrate that Ohr functions directly as a hydroperoxide reductase, converting both inorganic and organic hydroperoxides to less toxic metabolites. Site-directed mutagenesis confirms that the two conserved cysteines in each active site are essential for catalytic activity. We propose that the Ohr catalytic mechanism is similar to that of the structurally unrelated peroxiredoxins, directly utilizing highly reactive cysteine thiol groups to elicit hydroperoxide reduction.
+<StructureSection load='1n2f' size='340' side='right'caption='[[1n2f]], [[Resolution|resolution]] 2.01&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1n2f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N2F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N2F FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.01&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n2f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n2f OCA], [https://pdbe.org/1n2f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n2f RCSB], [https://www.ebi.ac.uk/pdbsum/1n2f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n2f ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q9HZZ3_PSEAE Q9HZZ3_PSEAE]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n2/1n2f_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n2f ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-N2F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1] with <scene name='pdbligand=DTT:'>DTT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N2F OCA].
+*[[Organic hydroperoxide resistance protein|Organic hydroperoxide resistance protein]]
+__TOC__
-==Reference==
+</StructureSection>
-Structural and functional characterization of the Pseudomonas hydroperoxide resistance protein Ohr., Lesniak J, Barton WA, Nikolov DB, EMBO J. 2002 Dec 16;21(24):6649-59. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12485986 12485986]
+[[Category: Large Structures]]
-[[Category: Pseudomonas aeruginosa pao1]]
+[[Category: Pseudomonas aeruginosa PAO1]]
-[[Category: Single protein]]
+[[Category: Barton WA]]
-[[Category: Barton, W A.]]
+[[Category: Lesniak J]]
-[[Category: Lesniak, J.]]
+[[Category: Nikolov DB]]
-[[Category: Nikolov, D B.]]
-[[Category: DTT]]
-[[Category: peroxide reductase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:01:36 2008''