1n05: Difference between revisions

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-[[Image:1n05.gif|left|200px]]<br /><applet load="1n05" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1n05, resolution 2.1&Aring;" />
-'''Crystal Structure of Schizosaccharomyces pombe Riboflavin Kinase Reveals a Novel ATP and Riboflavin Binding Fold'''<br />
-==Overview==
+==Crystal Structure of Schizosaccharomyces pombe Riboflavin Kinase Reveals a Novel ATP and Riboflavin Binding Fold==
-The essential redox cofactors riboflavin monophosphate (FMN) and flavin, adenine dinucleotide (FAD) are synthesised from their precursor, riboflavin, in sequential reactions by the metal-dependent riboflavin, kinase and FAD synthetase. Here, we describe the 1.6A crystal structure of, the Schizosaccharomyces pombe riboflavin kinase. The enzyme represents a, novel family of phosphoryl transferring enzymes. It is a monomer, comprising a central beta-barrel clasped on one side by two C-terminal, helices that display an L-like shape. The opposite side of the beta-barrel, serves as a platform for substrate binding as demonstrated by complexes, with ADP and FMN. Formation of the ATP-binding site requires significant, rearrangements in a short alpha-helix as compared to the substrate free, form. The diphosphate moiety of ADP is covered by the glycine-rich flap I, formed from parts of this alpha-helix. In contrast, no significant changes, are observed upon binding of riboflavin. The ribityl side-chain might be, covered by a rather flexible flap II. The unusual metal-binding site, involves, in addition to the ADP phosphates, only the strictly conserved, Thr45. This may explain the preference for zinc observed in vitro.
+<StructureSection load='1n05' size='340' side='right'caption='[[1n05]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[1n05]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N05 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N05 FirstGlance]. <br>
-N05 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Active as [http://en.wikipedia.org/wiki/Riboflavin_kinase Riboflavin kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.26 2.7.1.26] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1N05 OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n05 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n05 OCA], [https://pdbe.org/1n05 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n05 RCSB], [https://www.ebi.ac.uk/pdbsum/1n05 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n05 ProSAT]</span></td></tr>
-==Reference==
+</table>
-Crystal structure of Schizosaccharomyces pombe riboflavin kinase reveals a novel ATP and riboflavin-binding fold., Bauer S, Kemter K, Bacher A, Huber R, Fischer M, Steinbacher S, J Mol Biol. 2003 Mar 7;326(5):1463-73. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12595258 12595258]
+== Function ==
-[[Category: Riboflavin kinase]]
+[https://www.uniprot.org/uniprot/RIFK_SCHPO RIFK_SCHPO] Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) coenzyme.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n0/1n05_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n05 ConSurf].
+<div style="clear:both"></div>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Schizosaccharomyces pombe]]
-[[Category: Single protein]]
+[[Category: Bacher A]]
-[[Category: Bacher, A.]]
+[[Category: Bauer S]]
-[[Category: Bauer, S.]]
+[[Category: Fischer M]]
-[[Category: Fischer, M.]]
+[[Category: Huber R]]
-[[Category: Huber, R.]]
+[[Category: Kemter K]]
-[[Category: Kemter, K.]]
+[[Category: Steinbacher S]]
-[[Category: Steinbacher, S.]]
-[[Category: flavin cofactors]]
-[[Category: kinase]]
-[[Category: metal binding]]
-[[Category: phosphoryl transferases]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:50:47 2007''