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[[Image:1mwh.jpg|left|200px]]


{{Structure
==REOVIRUS POLYMERASE LAMBDA3 BOUND TO MRNA CAP ANALOG==
|PDB= 1mwh |SIZE=350|CAPTION= <scene name='initialview01'>1mwh</scene>, resolution 2.50&Aring;
<StructureSection load='1mwh' size='340' side='right'caption='[[1mwh]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
|SITE=
== Structural highlights ==
|LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene> and <scene name='pdbligand=GTG:7-METHYL-GUANOSINE-5'-TRIPHOSPHATE-5'-GUANOSINE'>GTG</scene>
<table><tr><td colspan='2'>[[1mwh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Reovirus_sp. Reovirus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MWH FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
|GENE= L1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10891 Reovirus sp.])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTG:7-METHYL-GUANOSINE-5-TRIPHOSPHATE-5-GUANOSINE'>GTG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
}}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mwh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mwh OCA], [https://pdbe.org/1mwh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mwh RCSB], [https://www.ebi.ac.uk/pdbsum/1mwh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mwh ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RDRP_REOVD RDRP_REOVD] RNA-directed RNA polymerase that is involved in transcription and genome replication. Following infection, it catalyzes the synthesis of fully conservative plus strands. After core assembly, which consists in recruitment of one capped plus-strand for each genomic segments and polymerase complexes, the polymerase switches mode and catalyzes the synthesis of complementary minus-strands.


'''REOVIRUS POLYMERASE LAMBDA3 BOUND TO MRNA CAP ANALOG'''
==See Also==
 
*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
The reovirus polymerase and those of other dsRNA viruses function within the confines of a protein capsid to transcribe the tightly packed dsRNA genome segments. The crystal structure of the reovirus polymerase, lambda3, determined at 2.5 A resolution, shows a fingers-palm-thumb core, similar to those of other viral polymerases, surrounded by major N- and C-terminal elaborations, which create a cage-like structure, with four channels leading to the catalytic site. This "caged" polymerase has allowed us to visualize the results of several rounds of RNA polymerization directly in the crystals. A 5' cap binding site on the surface of lambda3 suggests a template retention mechanism by which attachment of the 5' end of the plus-sense strand facilitates insertion of the 3' end of the minus-sense strand into the template channel.
[[Category: Large Structures]]
 
[[Category: Reovirus sp]]
==About this Structure==
[[Category: Farsetta DL]]
1MWH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Reovirus_sp. Reovirus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWH OCA].
[[Category: Harrison SC]]
 
[[Category: Nibert ML]]
==Reference==
[[Category: Tao Y]]
RNA synthesis in a cage--structural studies of reovirus polymerase lambda3., Tao Y, Farsetta DL, Nibert ML, Harrison SC, Cell. 2002 Nov 27;111(5):733-45. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12464184 12464184]
[[Category: Reovirus sp.]]
[[Category: Single protein]]
[[Category: Farsetta, D L.]]
[[Category: Harrison, S C.]]
[[Category: Nibert, M L.]]
[[Category: Tao, Y.]]
[[Category: GTG]]
[[Category: MN]]
[[Category: polymerase]]
[[Category: polymerase-cap analog complex]]
[[Category: right hand configuration]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:49:06 2008''

Latest revision as of 10:49, 14 February 2024

REOVIRUS POLYMERASE LAMBDA3 BOUND TO MRNA CAP ANALOGREOVIRUS POLYMERASE LAMBDA3 BOUND TO MRNA CAP ANALOG

Structural highlights

1mwh is a 1 chain structure with sequence from Reovirus sp.. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.5Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RDRP_REOVD RNA-directed RNA polymerase that is involved in transcription and genome replication. Following infection, it catalyzes the synthesis of fully conservative plus strands. After core assembly, which consists in recruitment of one capped plus-strand for each genomic segments and polymerase complexes, the polymerase switches mode and catalyzes the synthesis of complementary minus-strands.

See Also

1mwh, resolution 2.50Å

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