1mwh: Difference between revisions

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-[[Image:1mwh.jpg|left|200px]]<br /><applet load="1mwh" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="1mwh, resolution 2.50&Aring;" />
-'''REOVIRUS POLYMERASE LAMBDA3 BOUND TO MRNA CAP ANALOG'''<br />
-==Overview==
+==REOVIRUS POLYMERASE LAMBDA3 BOUND TO MRNA CAP ANALOG==
-The reovirus polymerase and those of other dsRNA viruses function within the confines of a protein capsid to transcribe the tightly packed dsRNA genome segments. The crystal structure of the reovirus polymerase, lambda3, determined at 2.5 A resolution, shows a fingers-palm-thumb core, similar to those of other viral polymerases, surrounded by major N- and C-terminal elaborations, which create a cage-like structure, with four channels leading to the catalytic site. This "caged" polymerase has allowed us to visualize the results of several rounds of RNA polymerization directly in the crystals. A 5' cap binding site on the surface of lambda3 suggests a template retention mechanism by which attachment of the 5' end of the plus-sense strand facilitates insertion of the 3' end of the minus-sense strand into the template channel.
+<StructureSection load='1mwh' size='340' side='right'caption='[[1mwh]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1mwh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Reovirus_sp. Reovirus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MWH FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTG:7-METHYL-GUANOSINE-5-TRIPHOSPHATE-5-GUANOSINE'>GTG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mwh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mwh OCA], [https://pdbe.org/1mwh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mwh RCSB], [https://www.ebi.ac.uk/pdbsum/1mwh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mwh ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/RDRP_REOVD RDRP_REOVD] RNA-directed RNA polymerase that is involved in transcription and genome replication. Following infection, it catalyzes the synthesis of fully conservative plus strands. After core assembly, which consists in recruitment of one capped plus-strand for each genomic segments and polymerase complexes, the polymerase switches mode and catalyzes the synthesis of complementary minus-strands.
-==About this Structure==
+==See Also==
-MWH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Reovirus_sp. Reovirus sp.] with <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=GTG:'>GTG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWH OCA].
+*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-RNA synthesis in a cage--structural studies of reovirus polymerase lambda3., Tao Y, Farsetta DL, Nibert ML, Harrison SC, Cell. 2002 Nov 27;111(5):733-45. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12464184 12464184]
+[[Category: Large Structures]]
-[[Category: Reovirus sp.]]
+[[Category: Reovirus sp]]
-[[Category: Single protein]]
+[[Category: Farsetta DL]]
-[[Category: Farsetta, D L.]]
+[[Category: Harrison SC]]
-[[Category: Harrison, S C.]]
+[[Category: Nibert ML]]
-[[Category: Nibert, M L.]]
+[[Category: Tao Y]]
-[[Category: Tao, Y.]]
-[[Category: GTG]]
-[[Category: MN]]
-[[Category: polymerase]]
-[[Category: polymerase-cap analog complex]]
-[[Category: right hand configuration]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:59:44 2008''