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[[Image:1msv.gif|left|200px]]<br /><applet load="1msv" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1msv, resolution 1.75&Aring;" />
'''The S68A S-adenosylmethionine decarboxylase proenzyme processing mutant.'''<br />


==Overview==
==The S68A S-adenosylmethionine decarboxylase proenzyme processing mutant.==
S-Adenosylmethionine decarboxylase (AdoMetDC) is a pyruvoyl-dependent enzyme that catalyzes the formation of the aminopropyl group donor in the biosynthesis of the polyamines spermidine and spermine. The enzyme is synthesized as a protein precursor and is activated by an autocatalytic serinolysis reaction that creates the pyruvoyl group. The autoprocessing reaction proceeds via an N --&gt; O acyl rearrangement, generating first an oxyoxazolidine anion intermediate followed by an ester intermediate. A similar strategy is utilized in self-catalyzed protein splicing reactions and in autoproteolytic activation of protein precursors. Mutation of Ser68 to alanine in human AdoMetDC prevents processing by removing the serine side chain necessary for nucleophilic attack at the adjacent carbonyl carbon atom. We have determined the X-ray structure of the S68A mutant and have constructed models of the proenzyme and the oxyoxazolidine intermediate. Formation of the oxyoxazolidine intermediate is promoted by a hydrogen bond from Cys82 and stabilized by a hydrogen bond from Ser229. These observations are consistent with mutagenesis studies, which show that the C82S and C82A mutants process slowly and that the S229A mutant does not process at all. Donation of a proton by His243 to the nitrogen atom of the oxyoxazolidine ring converts the oxyoxazolidine anion to the ester intermediate. The absence of a base to activate the hydroxyl group of Ser68 suggests that strain may play a role in the cleavage reaction. Comparison of AdoMetDC with other self-processing proteins shows no common structural features. Comparison to histidine decarboxylase and aspartate decarboxylase shows that these pyruvoyl-dependent enzymes evolved different catalytic strategies for forming the same cofactor.
<StructureSection load='1msv' size='340' side='right'caption='[[1msv]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1msv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MSV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MSV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PUT:1,4-DIAMINOBUTANE'>PUT</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1msv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1msv OCA], [https://pdbe.org/1msv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1msv RCSB], [https://www.ebi.ac.uk/pdbsum/1msv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1msv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DCAM_HUMAN DCAM_HUMAN]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ms/1msv_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1msv ConSurf].
<div style="clear:both"></div>


==Disease==
==See Also==
Known disease associated with this structure: Acromesomelic dysplasia, Maroteaux type OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=108961 108961]]
*[[SAM decarboxylase|SAM decarboxylase]]
 
__TOC__
==About this Structure==
</StructureSection>
1MSV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=PUT:'>PUT</scene> and <scene name='pdbligand=TRS:'>TRS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Adenosylmethionine_decarboxylase Adenosylmethionine decarboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.50 4.1.1.50] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MSV OCA].
 
==Reference==
Mechanism of human S-adenosylmethionine decarboxylase proenzyme processing as revealed by the structure of the S68A mutant., Tolbert WD, Zhang Y, Cottet SE, Bennett EM, Ekstrom JL, Pegg AE, Ealick SE, Biochemistry. 2003 Mar 4;42(8):2386-95. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12600205 12600205]
[[Category: Adenosylmethionine decarboxylase]]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Bennett, E M.]]
[[Category: Bennett EM]]
[[Category: Cottet, S E.]]
[[Category: Cottet SE]]
[[Category: Ealick, S E.]]
[[Category: Ealick SE]]
[[Category: Ekstrom, J L.]]
[[Category: Ekstrom JL]]
[[Category: Pegg, A E.]]
[[Category: Pegg AE]]
[[Category: Tolbert, W D.]]
[[Category: Tolbert WD]]
[[Category: Zhang, Y.]]
[[Category: Zhang Y]]
[[Category: PUT]]
[[Category: TRS]]
[[Category: allosteric enzyme]]
[[Category: decarboxylase]]
[[Category: lyase]]
[[Category: pyruvate]]
[[Category: pyruvoyl]]
[[Category: s-adenosylmethionine]]
[[Category: sandwich]]
[[Category: spermidine biosynthesis]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:58:40 2008''

Latest revision as of 10:48, 14 February 2024

The S68A S-adenosylmethionine decarboxylase proenzyme processing mutant.The S68A S-adenosylmethionine decarboxylase proenzyme processing mutant.

Structural highlights

1msv is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.75Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

DCAM_HUMAN

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1msv, resolution 1.75Å

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OCA
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