1mqw: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1mqw.jpg|left|200px]]<br /><applet load="1mqw" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="1mqw, resolution 2.3&Aring;" />
-'''Structure of the MT-ADPRase in complex with three Mn2+ ions and AMPCPR, a Nudix enzyme'''<br />
-==Overview==
+==Structure of the MT-ADPRase in complex with three Mn2+ ions and AMPCPR, a Nudix enzyme==
-Nudix hydrolases are a family of proteins that contain the characteristic sequence GX(5)EX(7)REUXEEXG(I/L/V), the Nudix box. They catalyze the hydrolysis of a variety of nucleoside diphosphate derivatives such as ADP-ribose, Ap(n)A (3 &lt;/= n &lt;/= 6), NADH, and dATP. A number of Nudix hydrolases from several species, ranging from bacteria to humans, have been characterized, including, in some cases, the determination of their three-dimensional structures. The product of the Rv1700 gene of M. tuberculosis is a Nudix hydrolase specific for ADP-ribose (ADPR). We have determined the crystal structures of MT-ADPRase alone, and in complex with substrate, with substrate and the nonactivating metal ion Gd(3+), and in complex with a nonhydrolyzable ADPR analog and the activating metal ion Mn(2+). These structures, refined with data extending to resolutions between 2.0 and 2.3 A, showed that there are sequence differences in binding site residues between MT-ADPRase and a human homolog that may be exploited for antituberculosis drug development.
+<StructureSection load='1mqw' size='340' side='right'caption='[[1mqw]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1mqw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MQW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MQW FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADV:ALPHA-BETA+METHYLENE+ADP-RIBOSE'>ADV</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mqw OCA], [https://pdbe.org/1mqw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mqw RCSB], [https://www.ebi.ac.uk/pdbsum/1mqw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mqw ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/O33199_MYCTO O33199_MYCTO]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mq/1mqw_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mqw ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-MQW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=ADV:'>ADV</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/ADP-ribose_diphosphatase ADP-ribose diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.13 3.6.1.13] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MQW OCA].
+*[[ADP-ribose pyrophosphatase 3D structures|ADP-ribose pyrophosphatase 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-Structure and mechanism of MT-ADPRase, a nudix hydrolase from Mycobacterium tuberculosis., Kang LW, Gabelli SB, Cunningham JE, O'Handley SF, Amzel LM, Structure. 2003 Aug;11(8):1015-23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12906832 12906832]
+[[Category: Large Structures]]
-[[Category: ADP-ribose diphosphatase]]
 [[Category: Mycobacterium tuberculosis]]
-[[Category: Single protein]]
+[[Category: Amzel LM]]
-[[Category: Amzel, L M.]]
+[[Category: Bianchet MA]]
-[[Category: Bianchet, M A.]]
+[[Category: Cunningham JE]]
-[[Category: Cunningham, J E.]]
+[[Category: Gabelli SB]]
-[[Category: Gabelli, S B.]]
+[[Category: Kang L-W]]
-[[Category: Handley, S F.O.]]
+[[Category: O'Handley SF]]
-[[Category: Kang, L W.]]
-[[Category: ADV]]
-[[Category: MN]]
-[[Category: adpr]]
-[[Category: mycobacterium tuberculosis]]
-[[Category: nudix hydrolase]]
-[[Category: rv1700]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:58:06 2008''