1mpf: Difference between revisions

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New page: left|200px<br /><applet load="1mpf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mpf, resolution 3.0Å" /> '''STRUCTURAL AND FUNCTI...
 
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'''STRUCTURAL AND FUNCTIONAL ALTERATIONS OF A COLICIN RESISTANT MUTANT OF OMPF-PORIN FROM ESCHERICHIA COLI'''<br />


==Overview==
==STRUCTURAL AND FUNCTIONAL ALTERATIONS OF A COLICIN RESISTANT MUTANT OF OMPF-PORIN FROM ESCHERICHIA COLI==
A strain of Escherichia coli, selected on the basis of its resistance to, colicin N, reveals distinct structural and functional alterations in, unspecific OmpF porin. A single mutation [Gly-119--&gt;Asp (G119D)] was, identified in the internal loop L3 that contributes critically to the, formation of the construction inside the lumen of the pore. X-ray, structure analysis to a resolution of 3.0 A reveals a locally altered, peptide backbone, with the side chain of residue Asp-119 protruding into, the channel, causing the area of the constriction (7 x 11 A in the wild, type) to be subdivided into two intercommunicating subcompartments of 3-4, A in diameter. The functional consequences of this structural modification, consist of a reduction of the channel conductance by about one-third, of, altered ion selectivity and voltage gating, and of a decrease of, permeation rates of various sugars by factors of 2-12. The structural, modification of the mutant protein affects neither the beta-barrel, structure nor those regions of the molecule that are exposed at the cell, surface. Considering the colicin resistance of the mutant, it is inferred, that in vivo, colicin N traverses the outer membrane through the porin, channel or that the dynamics of the exposed loops are affected in the, mutant such that these may impede the binding of the toxin.
<StructureSection load='1mpf' size='340' side='right'caption='[[1mpf]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1mpf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MPF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MPF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mpf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mpf OCA], [https://pdbe.org/1mpf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mpf RCSB], [https://www.ebi.ac.uk/pdbsum/1mpf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mpf ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/OMPF_ECOLI OMPF_ECOLI] Forms pores that allow passive diffusion of small molecules across the outer membrane. It is also a receptor for the bacteriophage T2.<ref>PMID:19721064</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mp/1mpf_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mpf ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1MPF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with C8E as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MPF OCA].
*[[Porin 3D structures|Porin 3D structures]]
 
== References ==
==Reference==
<references/>
Structural and functional alterations of a colicin-resistant mutant of OmpF porin from Escherichia coli., Jeanteur D, Schirmer T, Fourel D, Simonet V, Rummel G, Widmer C, Rosenbusch JP, Pattus F, Pages JM, Proc Natl Acad Sci U S A. 1994 Oct 25;91(22):10675-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7524100 7524100]
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Schirmer, T.]]
[[Category: Schirmer T]]
[[Category: C8E]]
[[Category: membrane protein]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:36:29 2007''

Latest revision as of 10:46, 14 February 2024

STRUCTURAL AND FUNCTIONAL ALTERATIONS OF A COLICIN RESISTANT MUTANT OF OMPF-PORIN FROM ESCHERICHIA COLISTRUCTURAL AND FUNCTIONAL ALTERATIONS OF A COLICIN RESISTANT MUTANT OF OMPF-PORIN FROM ESCHERICHIA COLI

Structural highlights

1mpf is a 1 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

OMPF_ECOLI Forms pores that allow passive diffusion of small molecules across the outer membrane. It is also a receptor for the bacteriophage T2.[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ Duval V, Nicoloff H, Levy SB. Combined inactivation of lon and ycgE decreases multidrug susceptibility by reducing the amount of OmpF porin in Escherichia coli. Antimicrob Agents Chemother. 2009 Nov;53(11):4944-8. doi: 10.1128/AAC.00787-09., Epub 2009 Aug 31. PMID:19721064 doi:10.1128/AAC.00787-09

1mpf, resolution 3.00Å

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