1mow: Difference between revisions

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New page: left|200px<br /><applet load="1mow" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mow, resolution 2.40Å" /> '''E-DreI'''<br /> ==O...
 
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[[Image:1mow.gif|left|200px]]<br /><applet load="1mow" size="450" color="white" frame="true" align="right" spinBox="true"
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'''E-DreI'''<br />


==Overview==
==E-DreI==
We have generated an artificial highly specific endonuclease by fusing, domains of homing endonucleases I-DmoI and I-CreI and creating a new 1400, A(2) protein interface between these domains. Protein engineering was, accomplished by combining computational redesign and an in vivo, protein-folding screen. The resulting enzyme, E-DreI (Engineered, I-DmoI/I-CreI), binds a long chimeric DNA target site with nanomolar, affinity, cleaving it precisely at a rate equivalent to its natural, parents. The structure of an E-DreI/DNA complex demonstrates the accuracy, of the protein interface redesign algorithm and reveals how catalytic, function is maintained during the creation of the new endonuclease. These, results indicate that it may be possible to generate novel highly specific, DNA binding proteins from homing endonucleases.
<StructureSection load='1mow' size='340' side='right'caption='[[1mow]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1mow]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii] and [https://en.wikipedia.org/wiki/Desulfurococcus_mucosus Desulfurococcus mucosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MOW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MOW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mow FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mow OCA], [https://pdbe.org/1mow PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mow RCSB], [https://www.ebi.ac.uk/pdbsum/1mow PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mow ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DMO1_DESMO DMO1_DESMO] Endonuclease involved in intron homing. Recognizes a recognizes up to 20 bp of DNA in the 23S rRNA gene intron. It has a slow turnover rate and cuts the coding strand with a slight preference over the non-coding strand.[https://www.uniprot.org/uniprot/DNE1_CHLRE DNE1_CHLRE] Endonuclease involved in group I intron homing. Recognizes and cleaves a 19-24 bp palindromic DNA site.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mo/1mow_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mow ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1MOW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Desulfurococcus_mobilis_and_chlamydomonas_reinhardtii Desulfurococcus mobilis and chlamydomonas reinhardtii] with MG, SO4 and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MOW OCA].
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Design, activity, and structure of a highly specific artificial endonuclease., Chevalier BS, Kortemme T, Chadsey MS, Baker D, Monnat RJ, Stoddard BL, Mol Cell. 2002 Oct;10(4):895-905. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12419232 12419232]
[[Category: Chlamydomonas reinhardtii]]
[[Category: Desulfurococcus mobilis and chlamydomonas reinhardtii]]
[[Category: Desulfurococcus mucosus]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Baker, D.]]
[[Category: Baker D]]
[[Category: Chadsey, M.S.]]
[[Category: Chadsey MS]]
[[Category: Chevalier, B.S.]]
[[Category: Chevalier BS]]
[[Category: Jr., R.J.Monnat.]]
[[Category: Kortemme T]]
[[Category: Kortemme, T.]]
[[Category: Monnat Jr RJ]]
[[Category: Stoddard, B.L.]]
[[Category: Stoddard BL]]
[[Category: GOL]]
[[Category: MG]]
[[Category: SO4]]
[[Category: design]]
[[Category: endonuclease]]
[[Category: engineering]]
[[Category: homing]]
[[Category: laglidadg]]
[[Category: structure]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:35:48 2007''

Latest revision as of 10:46, 14 February 2024

E-DreIE-DreI

Structural highlights

1mow is a 12 chain structure with sequence from Chlamydomonas reinhardtii and Desulfurococcus mucosus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.4Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

DMO1_DESMO Endonuclease involved in intron homing. Recognizes a recognizes up to 20 bp of DNA in the 23S rRNA gene intron. It has a slow turnover rate and cuts the coding strand with a slight preference over the non-coding strand.DNE1_CHLRE Endonuclease involved in group I intron homing. Recognizes and cleaves a 19-24 bp palindromic DNA site.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1mow, resolution 2.40Å

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