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| [[Image:1mme.jpg|left|200px]]<br /><applet load="1mme" size="350" color="white" frame="true" align="right" spinBox="true"
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| caption="1mme, resolution 3.100Å" />
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| '''THE CRYSTAL STRUCTURE OF AN ALL-RNA HAMMERHEAD RIBOZYME: A PROPOSED MECHANISM FOR RNA CATALYTIC CLEAVAGE'''<br />
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| ==Overview== | | ==THE CRYSTAL STRUCTURE OF AN ALL-RNA HAMMERHEAD RIBOZYME: A PROPOSED MECHANISM FOR RNA CATALYTIC CLEAVAGE== |
| We have solved the crystal structure of an all-RNA hammerhead ribozyme having a single 2'-O-methyl cytosine incorporated at the active site to prevent cleavage. The conditions used differ from those in another recent solution in four significant ways: first, it is an all-RNA ribozyme rather than a DNA-RNA hybrid; second, the connectivity of the ribozyme backbone strands is different; third, the crystals were grown in the presence of a much lower concentration of salt; and fourth, the crystal packing scheme is very different. Nevertheless, the three-dimensional structure of the all-RNA hammerhead ribozyme is similar to the previous structure. Five potential Mg(II)-binding sites are identified, including one positioned near the ribozyme catalytic pocket. Upon this basis, as well as upon comparisons with the metal-binding sites in the structurally homologous uridine turn of tRNAPhe, we propose a mechanism for RNA catalytic cleavage.
| | <StructureSection load='1mme' size='340' side='right'caption='[[1mme]], [[Resolution|resolution]] 3.10Å' scene=''> |
| | == Structural highlights == |
| | <table><tr><td colspan='2'>[[1mme]] is a 4 chain structure. The May 2005 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Self-splicing RNA'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2005_5 10.2210/rcsb_pdb/mom_2005_5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MME OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MME FirstGlance]. <br> |
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1Å</td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mme OCA], [https://pdbe.org/1mme PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mme RCSB], [https://www.ebi.ac.uk/pdbsum/1mme PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mme ProSAT]</span></td></tr> |
| | </table> |
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| ==About this Structure== | | ==See Also== |
| 1MME is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. The following page contains interesting information on the relation of 1MME with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb65_1.html Self-splicing RNA]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MME OCA].
| | *[[Ribozyme 3D structures|Ribozyme 3D structures]] |
| | | __TOC__ |
| ==Reference==
| | </StructureSection> |
| The crystal structure of an all-RNA hammerhead ribozyme: a proposed mechanism for RNA catalytic cleavage., Scott WG, Finch JT, Klug A, Cell. 1995 Jun 30;81(7):991-1002. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7541315 7541315]
| | [[Category: Large Structures]] |
| [[Category: Protein complex]] | | [[Category: RCSB PDB Molecule of the Month]] |
| [[Category: Self-splicing RNA]] | | [[Category: Self-splicing RNA]] |
| [[Category: Finch, J T.]] | | [[Category: Finch JT]] |
| [[Category: Klug, A.]] | | [[Category: Klug A]] |
| [[Category: Scott, W G.]] | | [[Category: Scott WG]] |
| [[Category: catalytic rna]]
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| [[Category: loop]]
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| [[Category: rna hammerhead ribozyme]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:56:41 2008''
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