1mm8: Difference between revisions

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-{{Seed}}
-[[Image:1mm8.png|left|200px]]
-<!--
+==Crystal structure of Tn5 Transposase complexed with ME DNA==
-The line below this paragraph, containing "STRUCTURE_1mm8", creates the "Structure Box" on the page.
+<StructureSection load='1mm8' size='340' side='right'caption='[[1mm8]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1mm8]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MM8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MM8 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-{{STRUCTURE_1mm8|  PDB=1mm8  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mm8 OCA], [https://pdbe.org/1mm8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mm8 RCSB], [https://www.ebi.ac.uk/pdbsum/1mm8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mm8 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/TN5P_ECOLX TN5P_ECOLX] Mediates transposition of transposon Tn5 by a 'cut and paste' mechanism. First, the monomeric transposase binds the 19 bp inverted DNA repeats flanking the transposon. Then, dimerization of the DNA-bound transposase creates a synaptic DNA complex. After nicking of the first DNA strand, excision of the transposon proceeds through a series of intermediates. The transposase then mediates the insertion of the transposon at a new site by strand transfer. The activity of the wild-type transposase is very low, and is further inhibited by dimerization with the transposase inhibitor (inh).<ref>PMID:6260374</ref> <ref>PMID:6291786</ref> <ref>PMID:6303899</ref> <ref>PMID:1310499</ref> <ref>PMID:8226636</ref> <ref>PMID:8871560</ref> <ref>PMID:11877443</ref> <ref>PMID:12367522</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mm/1mm8_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mm8 ConSurf].
+<div style="clear:both"></div>
-===Crystal structure of Tn5 Transposase complexed with ME DNA===
+==See Also==
+*[[Transposase 3D structures|Transposase 3D structures]]
+== References ==
-<!--
+<references/>
-The line below this paragraph, {{ABSTRACT_PUBMED_12367522}}, adds the Publication Abstract to the page
+__TOC__
-(as it appears on PubMed at http://www.pubmed.gov), where 12367522 is the PubMed ID number.
+</StructureSection>
--->
-{{ABSTRACT_PUBMED_12367522}}
-==About this Structure==
-MM8 is a 3 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MM8 OCA].
-==Reference==
-<ref group="xtra">PMID:12367522</ref><references group="xtra"/>
 [[Category: Escherichia coli]]
-[[Category: Bhasin, A.]]
+[[Category: Large Structures]]
-[[Category: Lovell, S.]]
+[[Category: Bhasin A]]
-[[Category: Rayment, I.]]
+[[Category: Lovell S]]
-[[Category: Reznikoff, W S.]]
+[[Category: Rayment I]]
-[[Category: Steiniger-White, M.]]
+[[Category: Reznikoff WS]]
-[[Category: Protein-dna complex]]
+[[Category: Steiniger-White M]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 17:43:31 2009''