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[[Image:1mi7.gif|left|200px]]


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==Crystal Structure of Domain Swapped trp Aporepressor in 30%(v/v) Isopropanol==
The line below this paragraph, containing "STRUCTURE_1mi7", creates the "Structure Box" on the page.
<StructureSection load='1mi7' size='340' side='right'caption='[[1mi7]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1mi7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MI7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MI7 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene></td></tr>
{{STRUCTURE_1mi7|  PDB=1mi7  |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mi7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mi7 OCA], [https://pdbe.org/1mi7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mi7 RCSB], [https://www.ebi.ac.uk/pdbsum/1mi7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mi7 ProSAT]</span></td></tr>
 
</table>
'''Crystal Structure of Domain Swapped trp Aporepressor in 30%(v/v) Isopropanol'''
== Function ==
 
[https://www.uniprot.org/uniprot/TRPR_ECOLI TRPR_ECOLI] This protein is an aporepressor. When complexed with L-tryptophan it binds the operator region of the trp operon (5'-ACTAGT-'3') and prevents the initiation of transcription. The complex also regulates trp repressor biosynthesis by binding to its regulatory region.
 
== Evolutionary Conservation ==
==Overview==
[[Image:Consurf_key_small.gif|200px|right]]
The E. coli trp repressor (trpR) homodimer recognizes its palindromic DNA binding site through a pair of flexible helix-turn-helix (HTH) motifs displayed on an intertwined helical core. Flexible N-terminal arms mediate association between dimers bound to tandem DNA sites. The 2.5 A X-ray structure of trpR crystallized in 30% (v/v) isopropanol reveals a substantial conformational rearrangement of HTH motifs and N-terminal arms, with the protein appearing in the unusual form of an ordered 3D domain-swapped supramolecular array. Small angle X-ray scattering measurements show that the self-association properties of trpR in solution are fundamentally altered by isopropanol.
Check<jmol>
 
  <jmolCheckbox>
==About this Structure==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mi/1mi7_consurf.spt"</scriptWhenChecked>
1MI7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MI7 OCA].  
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==Reference==
  </jmolCheckbox>
E. coli trp repressor forms a domain-swapped array in aqueous alcohol., Lawson CL, Benoff B, Berger T, Berman HM, Carey J, Structure. 2004 Jun;12(6):1099-108. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15274929 15274929]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mi7 ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Benoff, B.]]
[[Category: Benoff B]]
[[Category: Berger, T.]]
[[Category: Berger T]]
[[Category: Berman, H M.]]
[[Category: Berman HM]]
[[Category: Carey, J.]]
[[Category: Carey J]]
[[Category: Lawson, C L.]]
[[Category: Lawson CL]]
[[Category: Alcohol induced conformational rearrangement]]
[[Category: Dna binding protein]]
[[Category: Domain swapping]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 01:05:59 2008''

Latest revision as of 10:44, 14 February 2024

Crystal Structure of Domain Swapped trp Aporepressor in 30%(v/v) IsopropanolCrystal Structure of Domain Swapped trp Aporepressor in 30%(v/v) Isopropanol

Structural highlights

1mi7 is a 1 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.5Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TRPR_ECOLI This protein is an aporepressor. When complexed with L-tryptophan it binds the operator region of the trp operon (5'-ACTAGT-'3') and prevents the initiation of transcription. The complex also regulates trp repressor biosynthesis by binding to its regulatory region.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1mi7, resolution 2.50Å

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