1mg4: Difference between revisions

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New page: left|200px<br /> <applet load="1mg4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mg4, resolution 1.504Å" /> '''STRUCTURE OF N-TER...
 
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[[Image:1mg4.gif|left|200px]]<br />
<applet load="1mg4" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1mg4, resolution 1.504&Aring;" />
'''STRUCTURE OF N-TERMINAL DOUBLECORTIN DOMAIN FROM DCLK: WILD TYPE PROTEIN'''<br />


==Overview==
==STRUCTURE OF N-TERMINAL DOUBLECORTIN DOMAIN FROM DCLK: WILD TYPE PROTEIN==
The doublecortin-like domains (DCX), which typically occur in tandem, are, novel microtubule-binding modules. DCX tandems are found in doublecortin, a 360-residue protein expressed in migrating neurons; the, doublecortin-like kinase (DCLK); the product of the RP1 gene that is, responsible for a form of inherited blindness; and several other proteins., Mutations in the gene encoding doublecortin cause lissencephaly in males, and the 'double-cortex syndrome' in females. We here report a solution, structure of the N-terminal DCX domain of human doublecortin and a 1.5 A, resolution crystal structure of the equivalent domain from human DCLK., Both show a stable, ubiquitin-like tertiary fold with distinct structural, similarities to GTPase-binding domains. We also show that the C-terminal, DCX domains of both proteins are only partially folded. In functional, assays, the N-terminal DCX domain of doublecortin binds only to assembled, microtubules, whereas the C-terminal domain binds to both microtubules and, unpolymerized tubulin.
<StructureSection load='1mg4' size='340' side='right'caption='[[1mg4]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1mg4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MG4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MG4 FirstGlance]. <br>
1MG4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MG4 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.504&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mg4 OCA], [https://pdbe.org/1mg4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mg4 RCSB], [https://www.ebi.ac.uk/pdbsum/1mg4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mg4 ProSAT]</span></td></tr>
The DCX-domain tandems of doublecortin and doublecortin-like kinase., Kim MH, Cierpicki T, Derewenda U, Krowarsch D, Feng Y, Devedjiev Y, Dauter Z, Walsh CA, Otlewski J, Bushweller JH, Derewenda ZS, Nat Struct Biol. 2003 May;10(5):324-33. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12692530 12692530]
</table>
== Function ==
[https://www.uniprot.org/uniprot/DCLK1_HUMAN DCLK1_HUMAN] Probable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mg/1mg4_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mg4 ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Bushweller, J.H.]]
[[Category: Bushweller JH]]
[[Category: Cierpickil, T.]]
[[Category: Cierpickil T]]
[[Category: Dauter, Z.]]
[[Category: Dauter Z]]
[[Category: Derewenda, U.]]
[[Category: Derewenda U]]
[[Category: Derewenda, Z.]]
[[Category: Derewenda Z]]
[[Category: Devedjiev, Y.]]
[[Category: Devedjiev Y]]
[[Category: Feng, Y.]]
[[Category: Feng Y]]
[[Category: Kim, M.H.]]
[[Category: Kim MH]]
[[Category: Krowarsch, D.]]
[[Category: Krowarsch D]]
[[Category: Otlewski, J.]]
[[Category: Otlewski J]]
[[Category: Walsh, C.A.]]
[[Category: Walsh CA]]
[[Category: SO4]]
[[Category: cortex development]]
[[Category: dcx domain]]
[[Category: doublecortin-like kinase]]
[[Category: microtubule bundling]]
[[Category: x-ray structure]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 18:10:57 2007''

Latest revision as of 10:43, 14 February 2024

STRUCTURE OF N-TERMINAL DOUBLECORTIN DOMAIN FROM DCLK: WILD TYPE PROTEINSTRUCTURE OF N-TERMINAL DOUBLECORTIN DOMAIN FROM DCLK: WILD TYPE PROTEIN

Structural highlights

1mg4 is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.504Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

DCLK1_HUMAN Probable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1mg4, resolution 1.50Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
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