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[[Image:1mb0.gif|left|200px]]


{{Structure
==CRYSTAL STRUCTURE OF THE RESPONSE REGULATOR DIVK AT PH 8.0 IN COMPLEX WITH MN2+==
|PDB= 1mb0 |SIZE=350|CAPTION= <scene name='initialview01'>1mb0</scene>, resolution 2.00&Aring;
<StructureSection load='1mb0' size='340' side='right'caption='[[1mb0]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>
<table><tr><td colspan='2'>[[1mb0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Caulobacter_vibrioides Caulobacter vibrioides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MB0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MB0 FirstGlance]. <br>
|ACTIVITY=
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
|GENE= divk ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=155892 Caulobacter vibrioides])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mb0 OCA], [https://pdbe.org/1mb0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mb0 RCSB], [https://www.ebi.ac.uk/pdbsum/1mb0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mb0 ProSAT]</span></td></tr>
|RELATEDENTRY=[[1m5t|1M5T]], [[1mav|1MAV]], [[1mb3|1MB3]]
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mb0 OCA], [http://www.ebi.ac.uk/pdbsum/1mb0 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1mb0 RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/Q9A5I4_CAUVC Q9A5I4_CAUVC]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mb/1mb0_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mb0 ConSurf].
<div style="clear:both"></div>


'''CRYSTAL STRUCTURE OF THE RESPONSE REGULATOR DIVK AT PH 8.0 IN COMPLEX WITH MN2+'''
==See Also==
 
*[[Response regulator 3D structure|Response regulator 3D structure]]
 
__TOC__
==Overview==
</StructureSection>
DivK is an essential response regulator in the Gram-negative bacterium Caulobacter crescentus and functions in a complex phosphorelay system that precisely controls the sequence of developmental events during the cell division cycle. Structure determinations of this single domain response regulator at different pH values demonstrated that the five-stranded alpha/beta fold of the DivK protein is fully defined only at acidic pH. The crystal structures of the apoprotein and of metal-bound DivK complexes at higher pH values revealed a synergistic pH- and cation binding-induced flexibility of the beta4-alpha4 loop and of the alpha4 helix. This motion increases the solvent accessibility of the single cysteine residue in the protein. Solution state studies demonstrated a 200-fold pH-dependent increase in the affinity of manganese for the protein between pH 6.0 and 8.5 that seems to involve deprotonation of an acido-basic couple. Taken together, these results suggest that flexibility of critical regions of the protein, ionization of the cysteine 99 residue and improved K(D) values for the catalytic metal ion are coupled events. We propose that the molecular events observed in the isolated protein may be required for DivK activation and that they may be achieved in vivo through the specific protein-protein interactions between the response regulator and its cognate kinases.
 
==About this Structure==
1MB0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Caulobacter_vibrioides Caulobacter vibrioides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MB0 OCA].
 
==Reference==
Crystallographic and biochemical studies of DivK reveal novel features of an essential response regulator in Caulobacter crescentus., Guillet V, Ohta N, Cabantous S, Newton A, Samama JP, J Biol Chem. 2002 Nov 1;277(44):42003-10. Epub 2002 Aug 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12176983 12176983]
[[Category: Caulobacter vibrioides]]
[[Category: Caulobacter vibrioides]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Cabantous, S.]]
[[Category: Cabantous S]]
[[Category: Guillet, V.]]
[[Category: Guillet V]]
[[Category: Newton, A.]]
[[Category: Newton A]]
[[Category: Ohta, N.]]
[[Category: Ohta N]]
[[Category: SPINE, Structural Proteomics in Europe.]]
[[Category: Samama J-P]]
[[Category: Samama, J-P.]]
[[Category: response regulator]]
[[Category: signal transduction protein]]
[[Category: spine]]
[[Category: structural genomic]]
[[Category: structural proteomics in europe]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:13:47 2008''

Latest revision as of 10:42, 14 February 2024

CRYSTAL STRUCTURE OF THE RESPONSE REGULATOR DIVK AT PH 8.0 IN COMPLEX WITH MN2+CRYSTAL STRUCTURE OF THE RESPONSE REGULATOR DIVK AT PH 8.0 IN COMPLEX WITH MN2+

Structural highlights

1mb0 is a 1 chain structure with sequence from Caulobacter vibrioides. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9A5I4_CAUVC

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1mb0, resolution 2.00Å

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