1mas: Difference between revisions

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OCA

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-[[Image:1mas.gif|left|200px]]<br />
-<applet load="1mas" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1mas, resolution 2.5&Aring;" />
-'''PURINE NUCLEOSIDE HYDROLASE'''<br />
-==Overview==
+==PURINE NUCLEOSIDE HYDROLASE==
-Protozoan parasites rely on the host for purines since they lack a de novo, synthetic pathway. Crithidia fasciculata salvages exogenous inosine, primarily through hydrolysis of the N-ribosidic bond using several, nucleoside hydrolases. The most abundant nucleoside hydrolase is, relatively nonspecific but prefers inosine and uridine as substrates. Here, we report the three-dimensional structure of the inosine-uridine, nucleoside hydrolase (IU-NH) from C. fasciculata determined by X-ray, crystallography at a nominal resolution of 2.5 A. The enzyme has an open, (alpha, beta) structure which differs from the classical dinucleotide, binding fold. IU-nucleoside hydrolase is composed of a mixed, eight-stranded beta sheet surrounded by six alpha helices and a small, C-terminal lobe composed of ... [[http://ispc.weizmann.ac.il/pmbin/getpm?8634238 (full description)]]
+<StructureSection load='1mas' size='340' side='right'caption='[[1mas]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[1mas]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Crithidia_fasciculata Crithidia fasciculata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MAS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MAS FirstGlance]. <br>
-MAS is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Crithidia_fasciculata Crithidia fasciculata]] with K as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.1 3.2.2.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MAS OCA]].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
-==Reference==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mas FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mas OCA], [https://pdbe.org/1mas PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mas RCSB], [https://www.ebi.ac.uk/pdbsum/1mas PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mas ProSAT]</span></td></tr>
-Three-dimensional structure of the inosine-uridine nucleoside N-ribohydrolase from Crithidia fasciculata., Degano M, Gopaul DN, Scapin G, Schramm VL, Sacchettini JC, Biochemistry. 1996 May 14;35(19):5971-81. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8634238 8634238]
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/IUNH_CRIFA IUNH_CRIFA] Catalyzes the hydrolysis of all of the commonly occurring purine and pyrimidine nucleosides into ribose and the associated base, but has a preference for inosine and uridine as substrates.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ma/1mas_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mas ConSurf].
+<div style="clear:both"></div>
+__TOC__
+</StructureSection>
 [[Category: Crithidia fasciculata]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Degano, M.]]
+[[Category: Degano M]]
-[[Category: Gopaul, D.N.]]
+[[Category: Gopaul DN]]
-[[Category: Sacchettini, J.C.]]
+[[Category: Sacchettini JC]]
-[[Category: Scapin, G.]]
+[[Category: Scapin G]]
-[[Category: Schramm, V.L.]]
+[[Category: Schramm VL]]
-[[Category: K]]
-[[Category: hydrolase]]
-[[Category: iu-nh]]
-[[Category: purine nucleosidase]]
-[[Category: purine nucleoside hydrolase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 18:52:44 2007''