1m93: Difference between revisions

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New page: left|200px<br /><applet load="1m93" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m93, resolution 1.65Å" /> '''1.65 A Structure of ...
 
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[[Image:1m93.gif|left|200px]]<br /><applet load="1m93" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1m93, resolution 1.65&Aring;" />
'''1.65 A Structure of Cleaved Viral Serpin CRMA'''<br />


==Overview==
==1.65 A Structure of Cleaved Viral Serpin CRMA==
CrmA is an unusual viral serpin that inhibits both cysteine and serine, proteinases involved in the regulation of host inflammatory and apoptosis, processes. It differs from other members of the serpin superfamily by, having a reactive center loop that is one residue shorter, and by its, apparent inability to form SDS-stable covalent complexes with cysteine, proteinases. To obtain insight into the inhibitory mechanism of crmA, we, determined the crystal structure of reactive center loop-cleaved crmA to, 2.9 A resolution. The structure, which is the first of a viral serpin, suggests that crmA can inhibit cysteine proteinases by a mechanism, analogous to that used by other serpins against serine proteinases., However, one striking difference from other serpins, which may be, significant for in vivo function, is an additional highly charged, antiparallel strand for b sheet A, whose sequence and length are unique to, crmA.
<StructureSection load='1m93' size='340' side='right'caption='[[1m93]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1m93]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Cowpox_virus Cowpox virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M93 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M93 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m93 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m93 OCA], [https://pdbe.org/1m93 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m93 RCSB], [https://www.ebi.ac.uk/pdbsum/1m93 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m93 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SPI2_CWPXB SPI2_CWPXB] Specific and potent inhibitor of the interleukin-1-beta converting enzyme (ICE) thereby suppressing an interleukin-1 beta response to infection. The inhibition of ICE by crmA is an example of a "cross-class" interaction, in which a serpin inhibits a non-serine proteinase. Also inhibits granzyme B.<ref>PMID:1339309</ref> <ref>PMID:8034697</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m9/1m93_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m93 ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1M93 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Cupixi_virus Cupixi virus] with PO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1M93 OCA].
*[[Serpin 3D structures|Serpin 3D structures]]
 
== References ==
==Reference==
<references/>
Crystal structure of viral serpin crmA provides insights into its mechanism of cysteine proteinase inhibition., Simonovic M, Gettins PGW, Volz K, Protein Sci. 2000 Aug;9(8):1423-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10975564 10975564]
__TOC__
[[Category: Cupixi virus]]
</StructureSection>
[[Category: Protein complex]]
[[Category: Cowpox virus]]
[[Category: Gettins, P.G.W.]]
[[Category: Large Structures]]
[[Category: Simonovic, M.]]
[[Category: Gettins PGW]]
[[Category: Volz, K.]]
[[Category: Simonovic M]]
[[Category: PO4]]
[[Category: Volz K]]
[[Category: apoptosis]]
[[Category: crma]]
[[Category: ice inhibitor]]
[[Category: serpin]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:14:54 2007''

Latest revision as of 10:41, 14 February 2024

1.65 A Structure of Cleaved Viral Serpin CRMA1.65 A Structure of Cleaved Viral Serpin CRMA

Structural highlights

1m93 is a 3 chain structure with sequence from Cowpox virus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.65Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SPI2_CWPXB Specific and potent inhibitor of the interleukin-1-beta converting enzyme (ICE) thereby suppressing an interleukin-1 beta response to infection. The inhibition of ICE by crmA is an example of a "cross-class" interaction, in which a serpin inhibits a non-serine proteinase. Also inhibits granzyme B.[1] [2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ Ray CA, Black RA, Kronheim SR, Greenstreet TA, Sleath PR, Salvesen GS, Pickup DJ. Viral inhibition of inflammation: cowpox virus encodes an inhibitor of the interleukin-1 beta converting enzyme. Cell. 1992 May 15;69(4):597-604. PMID:1339309
  2. ↑ Komiyama T, Ray CA, Pickup DJ, Howard AD, Thornberry NA, Peterson EP, Salvesen G. Inhibition of interleukin-1 beta converting enzyme by the cowpox virus serpin CrmA. An example of cross-class inhibition. J Biol Chem. 1994 Jul 29;269(30):19331-7. PMID:8034697

1m93, resolution 1.65Å

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