1m7o: Difference between revisions

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New page: left|200px<br /><applet load="1m7o" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m7o, resolution 2.40Å" /> '''Plasmodium Falciparu...
 
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[[Image:1m7o.jpg|left|200px]]<br /><applet load="1m7o" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1m7o, resolution 2.40&Aring;" />
'''Plasmodium Falciparum Triosephosphate isomerase (PfTIM) compled to substrate analog 3-phosphoglycerate (3PG)'''<br />


==Overview==
==Plasmodium Falciparum Triosephosphate isomerase (PfTIM) compled to substrate analog 3-phosphoglycerate (3PG)==
The glycolytic enzymes of Plasmodium falciparum (Pf) are attractive drug, targets as the parasites lack a functional tricarboxylic cycle and hence, depend heavily on glycolysis for their energy requirements. Structural, comparisons between Pf triosephosphate isomerase (PfTIM) and its human, homologue have highlighted the important differences between the host and, parasite enzymes [Velanker et al. (1997), Structure, 5, 751-761]., Structures of various PfTIM-ligand complexes have been determined in order, to gain further insight into the mode of inhibitor binding to the parasite, enzyme. Structures of two PfTIM-substrate analogue complexes, those of, 3-phosphoglycerate (3PG) and glycerol-3-phosphate (G3P), have been, determined and refined at 2.4 A resolution. Both complexes crystallized in, the monoclinic space group P2(1), with a molecular dimer in the asymmetric, unit. The novel aspect of these structures is the adoption of the, 'loop-open' conformation, with the catalytic loop (loop 6, residues, 166-176) positioned away from the active site; this loop is known to move, by about 7 A towards the active site upon inhibitor binding in other TIMs., The loop-open form in the PfTIM complexes appears to be a consequence of, the S96F mutation, which is specific to the enzymes from malarial, parasites. Structural comparison with the corresponding complexes of, Trypanosoma brucei TIM (TrypTIM) shows that extensive steric clashes may, be anticipated between Phe96 and Ile172 in the 'closed' conformation of, the catalytic loop, preventing loop closure in PfTIM. Ser73 in PfTIM (Ala, in all other known TIMs) appears to provide an anchoring water-mediated, hydrogen bond to the ligand, compensating for the loss of a stabilizing, hydrogen bond from Gly171 NH in the closed-loop liganded TIM structures.
<StructureSection load='1m7o' size='340' side='right'caption='[[1m7o]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1m7o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M7O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M7O FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3PG:3-PHOSPHOGLYCERIC+ACID'>3PG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m7o OCA], [https://pdbe.org/1m7o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m7o RCSB], [https://www.ebi.ac.uk/pdbsum/1m7o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m7o ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TPIS_PLAFA TPIS_PLAFA]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m7/1m7o_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m7o ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1M7O is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum] with 3PG as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1M7O OCA].
*[[Triose phosphate isomerase 3D structures|Triose phosphate isomerase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Structures of Plasmodium falciparum triosephosphate isomerase complexed to substrate analogues: observation of the catalytic loop in the open conformation in the ligand-bound state., Parthasarathy S, Balaram H, Balaram P, Murthy MR, Acta Crystallogr D Biol Crystallogr. 2002 Dec;58(Pt 12):1992-2000. Epub, 2002 Nov 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12454456 12454456]
[[Category: Large Structures]]
[[Category: Plasmodium falciparum]]
[[Category: Plasmodium falciparum]]
[[Category: Single protein]]
[[Category: Balaram H]]
[[Category: Triose-phosphate isomerase]]
[[Category: Balaram P]]
[[Category: Balaram, H.]]
[[Category: Murthy MRN]]
[[Category: Balaram, P.]]
[[Category: Parthasarathy S]]
[[Category: Murthy, M.R.N.]]
[[Category: Parthasarathy, S.]]
[[Category: 3PG]]
[[Category: tim barrels; beta-alpha barrels; enzyme-inhibitor complex]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:12:26 2007''

Latest revision as of 10:41, 14 February 2024

Plasmodium Falciparum Triosephosphate isomerase (PfTIM) compled to substrate analog 3-phosphoglycerate (3PG)Plasmodium Falciparum Triosephosphate isomerase (PfTIM) compled to substrate analog 3-phosphoglycerate (3PG)

Structural highlights

1m7o is a 2 chain structure with sequence from Plasmodium falciparum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.4Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TPIS_PLAFA

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1m7o, resolution 2.40Å

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
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