1m7h: Difference between revisions

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[[Image:1m7h.jpg|left|200px]]


{{Structure
==Crystal Structure of APS kinase from Penicillium Chrysogenum: Structure with APS soaked out of one dimer==
|PDB= 1m7h |SIZE=350|CAPTION= <scene name='initialview01'>1m7h</scene>, resolution 2.00&Aring;
<StructureSection load='1m7h' size='340' side='right'caption='[[1m7h]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene> and <scene name='pdbligand=ADX:ADENOSINE-5'-PHOSPHOSULFATE'>ADX</scene>
<table><tr><td colspan='2'>[[1m7h]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Penicillium_chrysogenum Penicillium chrysogenum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M7H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M7H FirstGlance]. <br>
|ACTIVITY= [http://en.wikipedia.org/wiki/Adenylyl-sulfate_kinase Adenylyl-sulfate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.25 2.7.1.25]  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
|GENE=
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ADX:ADENOSINE-5-PHOSPHOSULFATE'>ADX</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
}}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m7h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m7h OCA], [https://pdbe.org/1m7h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m7h RCSB], [https://www.ebi.ac.uk/pdbsum/1m7h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m7h ProSAT]</span></td></tr>
 
</table>
'''Crystal Structure of APS kinase from Penicillium Chrysogenum: Structure with APS soaked out of one dimer'''
== Function ==
 
[https://www.uniprot.org/uniprot/KAPS_PENCH KAPS_PENCH] Catalyzes the synthesis of activated sulfate.
 
== Evolutionary Conservation ==
==Overview==
[[Image:Consurf_key_small.gif|200px|right]]
Adenosine 5'-phosphosulfate (APS) kinase catalyzes the second reaction in the two-step, ATP-dependent conversion of inorganic sulfate to 3'-phosphoadenosine 5'-phosphosulfate (PAPS). PAPS serves as the sulfuryl donor for the biosynthesis of all sulfate esters and also as a precursor of reduced sulfur biomolecules in many organisms. Previously, we determined the crystal structure of ligand-free APS kinase from the filamentous fungus, Penicillium chrysogenum [MacRae et al. (2000) Biochemistry 39, 1613-1621]. That structure contained a protease-susceptible disordered region ("mobile lid"; residues 145-170). Addition of MgADP and APS, which together promote the formation of a nonproductive "dead-end" ternary complex, protected the lid from trypsin. This report presents the 1.43 A resolution crystal structure of APS kinase with both ADP and APS bound at the active site and the 2.0 A resolution structure of the enzyme with ADP alone bound. The mobile lid is ordered in both complexes and is shown to provide part of the binding site for APS. That site is formed primarily by the highly conserved Arg 66, Arg 80, and Phe 75 from the protein core and Phe 165 from the mobile lid. The two Phe residues straddle the adenine ring of bound APS. Arg 148, a completely conserved residue, is the only residue in the mobile lid that interacts directly with bound ADP. Ser 34, located in the apex of the P-loop, hydrogen-bonds to the 3'-OH of APS, the phosphoryl transfer target. The structure of the binary E.ADP complex revealed further changes in the active site and N-terminal helix that occur upon the binding/release of (P)APS.
Check<jmol>
 
  <jmolCheckbox>
==About this Structure==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m7/1m7h_consurf.spt"</scriptWhenChecked>
1M7H is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Penicillium_chrysogenum Penicillium chrysogenum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M7H OCA].  
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==Reference==
  </jmolCheckbox>
Ligand-induced structural changes in adenosine 5'-phosphosulfate kinase from Penicillium chrysogenum., Lansdon EB, Segel IH, Fisher AJ, Biochemistry. 2002 Nov 19;41(46):13672-80. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12427029 12427029]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m7h ConSurf].
[[Category: Adenylyl-sulfate kinase]]
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Penicillium chrysogenum]]
[[Category: Penicillium chrysogenum]]
[[Category: Single protein]]
[[Category: Fisher AJ]]
[[Category: Fisher, A J.]]
[[Category: Lansdon EB]]
[[Category: Lansdon, E B.]]
[[Category: Sege IH]]
[[Category: Sege, I H.]]
[[Category: ADP]]
[[Category: ADX]]
[[Category: SO4]]
[[Category: adenylylsulfate kinase]]
[[Category: aps kinase]]
[[Category: nucleotide 2 kinase]]
[[Category: sulfate metabolism]]
[[Category: transferase]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:39:54 2008''

Latest revision as of 10:40, 14 February 2024

Crystal Structure of APS kinase from Penicillium Chrysogenum: Structure with APS soaked out of one dimerCrystal Structure of APS kinase from Penicillium Chrysogenum: Structure with APS soaked out of one dimer

Structural highlights

1m7h is a 4 chain structure with sequence from Penicillium chrysogenum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

KAPS_PENCH Catalyzes the synthesis of activated sulfate.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1m7h, resolution 2.00Å

Drag the structure with the mouse to rotate

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