1m7a: Difference between revisions

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New page: left|200px<br /><applet load="1m7a" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m7a, resolution 1.76Å" /> '''CANDIDA ALBICANS DIH...
 
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[[Image:1m7a.gif|left|200px]]<br /><applet load="1m7a" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1m7a, resolution 1.76&Aring;" />
'''CANDIDA ALBICANS DIHYDROFOLATE REDUCTASE COMPLEXED WITH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE (NADPH) AND 7-[2-methoxy-1-(methoxymethyl)ethyl]-7H-pyrrolo[3,2-f] quinazoline-1,3-diamine (GW557)'''<br />


==Overview==
==CANDIDA ALBICANS DIHYDROFOLATE REDUCTASE COMPLEXED WITH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE (NADPH) AND 7-[2-methoxy-1-(methoxymethyl)ethyl]-7H-pyrrolo[3,2-f] quinazoline-1,3-diamine (GW557)==
The recent rise in systemic fungal infections has created a need for the, development of new antifungal agents. As part of an effort to provide, therapeutically effective inhibitors of fungal dihydrofolate reductase, (DHFR), we have cloned, expressed, purified, crystallized, and determined, the three-dimensional structure of Candida albicans DHFR. The 192-residue, enzyme, which was expressed in Escherichia coli and purified by, methotrexate affinity and cation exchange chromatography, was 27%, identical to human DHFR. Crystals of C. albicans DHFR were grown as the, holoenzyme complex and as a ternary complex containing a, pyrroloquinazoline inhibitor. Both complexes crystallized with two, molecules in the asymmetric unit in space group P21. The final structures, had R-factors of 0.199 at 1.85-A resolution and 0.155 at 1.60-A, resolution, respectively. The enzyme fold was similar to that of bacterial, and vertebrate DHFR, and the binding of a nonselective, diaminopyrroloquinazoline inhibitor and the interactions of NADPH with, protein were typical of ligand binding to other DHFRs. However, the width, of the active site cleft of C. albicans DHFR was significantly larger than, that of the human enzyme, providing a basis for the design of potentially, selective inhibitors.
<StructureSection load='1m7a' size='340' side='right'caption='[[1m7a]], [[Resolution|resolution]] 1.76&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1m7a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M7A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M7A FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.76&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MQU:7-[2-METHOXY-1-(METHOXYMETHYL)ETHYL]-7H-PYRROLO[3,2-F]+QUINAZOLINE-1,3-DIAMINE'>MQU</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m7a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m7a OCA], [https://pdbe.org/1m7a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m7a RCSB], [https://www.ebi.ac.uk/pdbsum/1m7a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m7a ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DYR_CANAX DYR_CANAX] Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m7/1m7a_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m7a ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1M7A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans] with NDP and MQU as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1M7A OCA].
*[[Dihydrofolate reductase 3D structures|Dihydrofolate reductase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
X-ray crystallographic studies of Candida albicans dihydrofolate reductase. High resolution structures of the holoenzyme and an inhibited ternary complex., Whitlow M, Howard AJ, Stewart D, Hardman KD, Kuyper LF, Baccanari DP, Fling ME, Tansik RL, J Biol Chem. 1997 Nov 28;272(48):30289-98. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9374515 9374515]
[[Category: Candida albicans]]
[[Category: Candida albicans]]
[[Category: Dihydrofolate reductase]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Howard AJ]]
[[Category: Howard, A.J.]]
[[Category: Kuyper LF]]
[[Category: Kuyper, L.F.]]
[[Category: Whitlow M]]
[[Category: Whitlow, M.]]
[[Category: MQU]]
[[Category: NDP]]
[[Category: antifungal target]]
[[Category: oxidoreductase]]
[[Category: reductase]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:12:04 2007''

Latest revision as of 10:40, 14 February 2024

CANDIDA ALBICANS DIHYDROFOLATE REDUCTASE COMPLEXED WITH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE (NADPH) AND 7-[2-methoxy-1-(methoxymethyl)ethyl]-7H-pyrrolo[3,2-f] quinazoline-1,3-diamine (GW557)CANDIDA ALBICANS DIHYDROFOLATE REDUCTASE COMPLEXED WITH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE (NADPH) AND 7-[2-methoxy-1-(methoxymethyl)ethyl]-7H-pyrrolo[3,2-f] quinazoline-1,3-diamine (GW557)

Structural highlights

1m7a is a 2 chain structure with sequence from Candida albicans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.76Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

DYR_CANAX Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1m7a, resolution 1.76Å

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