1m79: Difference between revisions

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-[[Image:1m79.gif|left|200px]]<br /><applet load="1m79" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1m79, resolution 1.70&Aring;" />
-'''Candida albicans Dihydrofolate Reductase Complexed with Dihydro-Nicotinamide-Adenine-Dinucleotide Phosphate (NADPH) and 5-(4-methoxyphenoxy)-2,4-quinazolinediamine (GW1466)'''<br />
-==Overview==
+==Candida albicans Dihydrofolate Reductase Complexed with Dihydro-Nicotinamide-Adenine-Dinucleotide Phosphate (NADPH) and 5-(4-methoxyphenoxy)-2,4-quinazolinediamine (GW1466)==
-The recent rise in systemic fungal infections has created a need for the, development of new antifungal agents. As part of an effort to provide, therapeutically effective inhibitors of fungal dihydrofolate reductase, (DHFR), we have cloned, expressed, purified, crystallized, and determined, the three-dimensional structure of Candida albicans DHFR. The 192-residue, enzyme, which was expressed in Escherichia coli and purified by, methotrexate affinity and cation exchange chromatography, was 27%, identical to human DHFR. Crystals of C. albicans DHFR were grown as the, holoenzyme complex and as a ternary complex containing a, pyrroloquinazoline inhibitor. Both complexes crystallized with two, molecules in the asymmetric unit in space group P21. The final structures, had R-factors of 0.199 at 1.85-A resolution and 0.155 at 1.60-A, resolution, respectively. The enzyme fold was similar to that of bacterial, and vertebrate DHFR, and the binding of a nonselective, diaminopyrroloquinazoline inhibitor and the interactions of NADPH with, protein were typical of ligand binding to other DHFRs. However, the width, of the active site cleft of C. albicans DHFR was significantly larger than, that of the human enzyme, providing a basis for the design of potentially, selective inhibitors.
+<StructureSection load='1m79' size='340' side='right'caption='[[1m79]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1m79]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M79 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M79 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=MQ1:5-(4-METHOXYPHENOXY)-2,4-QUINAZOLINEDIAMINE'>MQ1</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m79 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m79 OCA], [https://pdbe.org/1m79 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m79 RCSB], [https://www.ebi.ac.uk/pdbsum/1m79 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m79 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/DYR_CANAX DYR_CANAX] Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m7/1m79_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m79 ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-M79 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans] with NDP, MQ1 and MES as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1M79 OCA].
+*[[Dihydrofolate reductase 3D structures|Dihydrofolate reductase 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-X-ray crystallographic studies of Candida albicans dihydrofolate reductase. High resolution structures of the holoenzyme and an inhibited ternary complex., Whitlow M, Howard AJ, Stewart D, Hardman KD, Kuyper LF, Baccanari DP, Fling ME, Tansik RL, J Biol Chem. 1997 Nov 28;272(48):30289-98. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9374515 9374515]
 [[Category: Candida albicans]]
-[[Category: Dihydrofolate reductase]]
+[[Category: Large Structures]]
-[[Category: Single protein]]
+[[Category: Howard AJ]]
-[[Category: Howard, A.J.]]
+[[Category: Kuyper LF]]
-[[Category: Kuyper, L.F.]]
+[[Category: Whitlow M]]
-[[Category: Whitlow, M.]]
-[[Category: MES]]
-[[Category: MQ1]]
-[[Category: NDP]]
-[[Category: antifungal target]]
-[[Category: oxidoreductase]]
-[[Category: reductase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:12:01 2007''