1m4d: Difference between revisions

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-[[Image:1m4d.jpg|left|200px]]
- {{Structure
+==Aminoglycoside 2'-N-acetyltransferase from Mycobacterium tuberculosis-Complex with Coenzyme A and Tobramycin==
-|PDB= 1m4d |SIZE=350|CAPTION= <scene name='initialview01'>1m4d</scene>, resolution 1.8&Aring;
+<StructureSection load='1m4d' size='340' side='right'caption='[[1m4d]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=TOY:TOBRAMYCIN'>TOY</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene> and <scene name='pdbligand=PAP:3&#39;-PHOSPHATE-ADENOSINE-5&#39;-DIPHOSPHATE'>PAP</scene>
+<table><tr><td colspan='2'>[[1m4d]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M4D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M4D FirstGlance]. <br>
-|ACTIVITY=
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
-|GENE=
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=PAP:3-PHOSPHATE-ADENOSINE-5-DIPHOSPHATE'>PAP</scene>, <scene name='pdbligand=TOY:TOBRAMYCIN'>TOY</scene></td></tr>
-}}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m4d OCA], [https://pdbe.org/1m4d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m4d RCSB], [https://www.ebi.ac.uk/pdbsum/1m4d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m4d ProSAT]</span></td></tr>
+</table>
-'''Aminoglycoside 2'-N-acetyltransferase from Mycobacterium tuberculosis-Complex with Coenzyme A and Tobramycin'''
+== Function ==
+[https://www.uniprot.org/uniprot/AAC2_MYCTU AAC2_MYCTU] Catalyzes the coenzyme A-dependent acetylation of the 2' hydroxyl or amino group of a broad spectrum of aminoglycosides. It confers resistance to aminoglycosides.
+== Evolutionary Conservation ==
-==Overview==
+[[Image:Consurf_key_small.gif|200px|right]]
-AAC(2')-Ic catalyzes the coenzyme A (CoA)-dependent acetylation of the 2' hydroxyl or amino group of a broad spectrum of aminoglycosides. The crystal structure of the AAC(2')-Ic from Mycobacterium tuberculosis has been determined in the apo enzyme form and in ternary complexes with CoA and either tobramycin, kanamycin A or ribostamycin, representing the first structures of an aminoglycoside acetyltransferase bound to a drug. The overall fold of AAC(2')-Ic places it in the GCN5-related N-acetyltransferase (GNAT) superfamily. Although the physiological function of AAC(2')-Ic is uncertain, a structural analysis of these high-affinity aminoglycoside complexes suggests that the enzyme may acetylate a key biosynthetic intermediate of mycothiol, the major reducing agent in mycobacteria, and participate in the regulation of cellular redox potential.
+Check<jmol>
+  <jmolCheckbox>
-==About this Structure==
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m4/1m4d_consurf.spt"</scriptWhenChecked>
-M4D is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M4D OCA].
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
-==Reference==
+  </jmolCheckbox>
-Aminoglycoside 2'-N-acetyltransferase from Mycobacterium tuberculosis in complex with coenzyme A and aminoglycoside substrates., Vetting MW, Hegde SS, Javid-Majd F, Blanchard JS, Roderick SL, Nat Struct Biol. 2002 Sep;9(9):653-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12161746 12161746]
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m4d ConSurf].
-[[Category: Mycobacterium tuberculosis]]
+<div style="clear:both"></div>
-[[Category: Single protein]]
+__TOC__
-[[Category: Blanchard, J S.]]
+</StructureSection>
-[[Category: Hegde, S S.]]
+[[Category: Large Structures]]
-[[Category: Javid-Majd, F.]]
+[[Category: Mycobacterium tuberculosis H37Rv]]
-[[Category: Roderick, S L.]]
+[[Category: Blanchard JS]]
-[[Category: Vetting, M W.]]
+[[Category: Hegde SS]]
-[[Category: COA]]
+[[Category: Javid-Majd F]]
-[[Category: PAP]]
+[[Category: Roderick SL]]
-[[Category: TOY]]
+[[Category: Vetting MW]]
-[[Category: coa binding motif]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 12:42:51 2008''