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[[Image:1m0i.png|left|200px]]


{{STRUCTURE_1m0i|  PDB=1m0i  |  SCENE=  }}
==Crystal Structure of Bacteriophage T7 Endonuclease I with a Wild-Type Active Site==
 
<StructureSection load='1m0i' size='340' side='right'caption='[[1m0i]], [[Resolution|resolution]] 2.55&Aring;' scene=''>
===Crystal Structure of Bacteriophage T7 Endonuclease I with a Wild-Type Active Site===
== Structural highlights ==
 
<table><tr><td colspan='2'>[[1m0i]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_T7 Escherichia phage T7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M0I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M0I FirstGlance]. <br>
{{ABSTRACT_PUBMED_12093751}}
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.55&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
==About this Structure==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m0i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m0i OCA], [https://pdbe.org/1m0i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m0i RCSB], [https://www.ebi.ac.uk/pdbsum/1m0i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m0i ProSAT]</span></td></tr>
[[1m0i]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M0I OCA].  
</table>
== Function ==
[https://www.uniprot.org/uniprot/ENDO_BPT7 ENDO_BPT7] Junction-resolving enzyme that selectively binds and cleaves four-way (Holliday) DNA junctions present after viral genomic replication. These intermediates are created during DNA repair, processing of stalled replication forks and homologous genetic recombination. Introduces two nicks on the two non-crossing strands, at 5' sides of the junction. Participates also together with gp6 in the degradation of host chromosome to provide nucleotides for phage DNA synthesis.<ref>PMID:12628932</ref> <ref>PMID:23207296</ref> <ref>PMID:3972821</ref> <ref>PMID:9236119</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m0/1m0i_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m0i ConSurf].
<div style="clear:both"></div>


==See Also==
==See Also==
*[[Endonuclease|Endonuclease]]
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:012093751</ref><references group="xtra"/>
__TOC__
[[Category: Enterobacteria phage t7]]
</StructureSection>
[[Category: Declais, A C.]]
[[Category: Escherichia phage T7]]
[[Category: Hadden, J M.]]
[[Category: Large Structures]]
[[Category: Lilley, D M.]]
[[Category: Declais AC]]
[[Category: Phillips, S E.]]
[[Category: Hadden JM]]
[[Category: Composite active site]]
[[Category: Lilley DM]]
[[Category: Domain swapped]]
[[Category: Phillips SE]]
[[Category: Holliday junction resolvase]]
[[Category: Homodimer]]
[[Category: Hydrolase]]

Latest revision as of 10:38, 14 February 2024

Crystal Structure of Bacteriophage T7 Endonuclease I with a Wild-Type Active SiteCrystal Structure of Bacteriophage T7 Endonuclease I with a Wild-Type Active Site

Structural highlights

1m0i is a 4 chain structure with sequence from Escherichia phage T7. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.55Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ENDO_BPT7 Junction-resolving enzyme that selectively binds and cleaves four-way (Holliday) DNA junctions present after viral genomic replication. These intermediates are created during DNA repair, processing of stalled replication forks and homologous genetic recombination. Introduces two nicks on the two non-crossing strands, at 5' sides of the junction. Participates also together with gp6 in the degradation of host chromosome to provide nucleotides for phage DNA synthesis.[1] [2] [3] [4]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ Declais AC, Fogg JM, Freeman AD, Coste F, Hadden JM, Phillips SE, Lilley DM. The complex between a four-way DNA junction and T7 endonuclease I. EMBO J. 2003 Mar 17;22(6):1398-409. PMID:12628932 doi:http://dx.doi.org/10.1093/emboj/cdg132
  2. ↑ Freeman AD, Declais AC, Lilley DM. The importance of the N-terminus of T7 endonuclease I in the interaction with DNA junctions. J Mol Biol. 2013 Jan 23;425(2):395-410. doi: 10.1016/j.jmb.2012.11.029. Epub 2012, Dec 1. PMID:23207296 doi:http://dx.doi.org/10.1016/j.jmb.2012.11.029
  3. ↑ Panayotatos N, Fontaine A. An endonuclease specific for single-stranded DNA selectively damages the genomic DNA and induces the SOS response. J Biol Chem. 1985 Mar 10;260(5):3173-7. PMID:3972821
  4. ↑ Parkinson MJ, Lilley DM. The junction-resolving enzyme T7 endonuclease I: quaternary structure and interaction with DNA. J Mol Biol. 1997 Jul 11;270(2):169-78. PMID:9236119 doi:http://dx.doi.org/10.1006/jmbi.1997.1128

1m0i, resolution 2.55Å

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