1lu3: Difference between revisions

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New page: left|200px<br /><applet load="1lu3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lu3, resolution 16.8Å" /> '''Separate Fitting of ...
 
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[[Image:1lu3.gif|left|200px]]<br /><applet load="1lu3" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1lu3, resolution 16.8&Aring;" />
'''Separate Fitting of the Anticodon Loop Region of tRNA (nucleotide 26-42) in the Low Resolution Cryo-EM Map of an EF-Tu Ternary Complex (GDP and Kirromycin) Bound to E. coli 70S Ribosome'''<br />


==Overview==
==Separate Fitting of the Anticodon Loop Region of tRNA (nucleotide 26-42) in the Low Resolution Cryo-EM Map of an EF-Tu Ternary Complex (GDP and Kirromycin) Bound to E. coli 70S Ribosome==
During the elongation cycle of protein biosynthesis, the specific amino, acid coded for by the mRNA is delivered by a complex that is comprised of, the cognate aminoacyl-tRNA, elongation factor Tu and GTP. As this ternary, complex binds to the ribosome, the anticodon end of the tRNA reaches the, decoding center in the 30S subunit. Here we present the cryo- electron, microscopy (EM) study of an Escherichia coli 70S ribosome-bound ternary, complex stalled with an antibiotic, kirromycin. In the cryo-EM map the, anticodon arm of the tRNA presents a new conformation that appears to, facilitate the initial codon-anticodon interaction. Furthermore, the elbow, region of the tRNA is seen to contact the GTPase-associated center on the, 50S subunit of the ribosome, suggesting an active role of the tRNA in the, transmission of the signal prompting the GTP hydrolysis upon codon, recognition.
<SX load='1lu3' size='340' side='right' viewer='molstar' caption='[[1lu3]], [[Resolution|resolution]] 16.80&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1lu3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LU3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LU3 FirstGlance]. <br>
1LU3 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LU3 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 16.8&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PSU:PSEUDOURIDINE-5-MONOPHOSPHATE'>PSU</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lu3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lu3 OCA], [https://pdbe.org/1lu3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lu3 RCSB], [https://www.ebi.ac.uk/pdbsum/1lu3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lu3 ProSAT]</span></td></tr>
Cryo-EM reveals an active role for aminoacyl-tRNA in the accommodation process., Valle M, Sengupta J, Swami NK, Grassucci RA, Burkhardt N, Nierhaus KH, Agrawal RK, Frank J, EMBO J. 2002 Jul 1;21(13):3557-67. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12093756 12093756]
</table>
[[Category: Protein complex]]
__TOC__
</SX>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Agrawal, R.K.]]
[[Category: Agrawal RK]]
[[Category: Burkhardt, N.]]
[[Category: Burkhardt N]]
[[Category: Frank, J.]]
[[Category: Frank J]]
[[Category: Grassucci, R.A.]]
[[Category: Grassucci RA]]
[[Category: Nierhaus, K.H.]]
[[Category: Nierhaus KH]]
[[Category: Sengupta, J.]]
[[Category: Sengupta J]]
[[Category: Swami, N.K.]]
[[Category: Swami NK]]
[[Category: Valle, M.]]
[[Category: Valle M]]
[[Category: 70s e.coli ribosome]]
[[Category: conformational change]]
[[Category: cryo-em]]
[[Category: ternary complex]]
[[Category: trna]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 03:22:48 2007''

Latest revision as of 10:36, 14 February 2024

Separate Fitting of the Anticodon Loop Region of tRNA (nucleotide 26-42) in the Low Resolution Cryo-EM Map of an EF-Tu Ternary Complex (GDP and Kirromycin) Bound to E. coli 70S RibosomeSeparate Fitting of the Anticodon Loop Region of tRNA (nucleotide 26-42) in the Low Resolution Cryo-EM Map of an EF-Tu Ternary Complex (GDP and Kirromycin) Bound to E. coli 70S Ribosome

1lu3, resolution 16.80Å

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