1lt8: Difference between revisions

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New page: left|200px<br /> <applet load="1lt8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lt8, resolution 2.05Å" /> '''Reduced Homo sapien...
 
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[[Image:1lt8.gif|left|200px]]<br />
<applet load="1lt8" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1lt8, resolution 2.05&Aring;" />
'''Reduced Homo sapiens Betaine-Homocysteine S-Methyltransferase in Complex with S-(delta-carboxybutyl)-L-Homocysteine'''<br />


==Overview==
==Reduced Homo sapiens Betaine-Homocysteine S-Methyltransferase in Complex with S-(delta-carboxybutyl)-L-Homocysteine==
Betaine-homocysteine methyl transferase (BHMT) catalyzes the synthesis of, methionine from betaine and homocysteine (Hcy), utilizing a zinc ion to, activate Hcy. BHMT is a key liver enzyme that is important for, homocysteine homeostasis. X-ray structures of human BHMT in its oxidized, (Zn-free) and reduced (Zn-replete) forms, the latter in complex with the, bisubstrate analog, S(delta-carboxybutyl)-L-homocysteine, were determined, at resolutions of 2.15 A and 2.05 A. BHMT is a (beta/alpha)(8) barrel that, is distorted to construct the substrate and metal binding sites. The zinc, binding sequences G-V/L-N-C and G-G-C-C are at the C termini of strands, beta6 and beta8. Oxidation to the Cys217-Cys299 disulfide and expulsion of, Zn are accompanied by local rearrangements. The structures identify Hcy, binding fingerprints and provide a prototype for the homocysteine, S-methyltransferase family.
<StructureSection load='1lt8' size='340' side='right'caption='[[1lt8]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1lt8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LT8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LT8 FirstGlance]. <br>
1LT8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ZN, CBH and CIT as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Betaine--homocysteine_S-methyltransferase Betaine--homocysteine S-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.5 2.1.1.5] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LT8 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CBH:S-(D-CARBOXYBUTYL)-L-HOMOCYSTEINE'>CBH</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lt8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lt8 OCA], [https://pdbe.org/1lt8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lt8 RCSB], [https://www.ebi.ac.uk/pdbsum/1lt8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lt8 ProSAT]</span></td></tr>
Betaine-homocysteine methyltransferase: zinc in a distorted barrel., Evans JC, Huddler DP, Jiracek J, Castro C, Millian NS, Garrow TA, Ludwig ML, Structure. 2002 Sep;10(9):1159-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12220488 12220488]
</table>
[[Category: Betaine--homocysteine S-methyltransferase]]
== Function ==
[https://www.uniprot.org/uniprot/BHMT1_HUMAN BHMT1_HUMAN] Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lt/1lt8_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lt8 ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Castro, C.]]
[[Category: Castro C]]
[[Category: Evans, J.C.]]
[[Category: Evans JC]]
[[Category: Garrow, T.A.]]
[[Category: Garrow TA]]
[[Category: Huddler, D.P.]]
[[Category: Huddler DP]]
[[Category: Jiracek, J.]]
[[Category: Jiracek J]]
[[Category: Ludwig, M.L.]]
[[Category: Ludwig ML]]
[[Category: Millian, N.S.]]
[[Category: Millian NS]]
[[Category: CBH]]
[[Category: CIT]]
[[Category: ZN]]
[[Category: homocysteine metabolism]]
[[Category: homocysteinemia]]
[[Category: thiol alkyl transfer]]
[[Category: transferase]]
[[Category: zinc]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 18:04:03 2007''

Latest revision as of 10:35, 14 February 2024

Reduced Homo sapiens Betaine-Homocysteine S-Methyltransferase in Complex with S-(delta-carboxybutyl)-L-HomocysteineReduced Homo sapiens Betaine-Homocysteine S-Methyltransferase in Complex with S-(delta-carboxybutyl)-L-Homocysteine

Structural highlights

1lt8 is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.05Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

BHMT1_HUMAN Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1lt8, resolution 2.05Å

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