1lnq: Difference between revisions

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OCA

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-[[Image:1lnq.gif|left|200px]]<br />
-<applet load="1lnq" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1lnq, resolution 3.30&Aring;" />
-'''CRYSTAL STRUCTURE OF MTHK AT 3.3 A'''<br />
-==Overview==
+==CRYSTAL STRUCTURE OF MTHK AT 3.3 A==
-Ion channels exhibit two essential biophysical properties; that is, selective ion conduction, and the ability to gate-open in response to an, appropriate stimulus. Two general categories of ion channel gating are, defined by the initiating stimulus: ligand binding (neurotransmitter- or, second-messenger-gated channels) or membrane voltage (voltage-gated, channels). Here we present the structural basis of ligand gating in a K(+), channel that opens in response to intracellular Ca(2+). We have cloned, expressed, analysed electrical properties, and determined the crystal, structure of a K(+) channel (MthK) from Methanobacterium, thermoautotrophicum in the Ca(2+)-bound, opened state. Eight RCK domains, (regulators of K(+) conductance) form a gating ring at the intracellular, membrane surface. The gating ring uses the free energy of Ca(2+) binding, in a simple manner to perform mechanical work to open the pore.
+<StructureSection load='1lnq' size='340' side='right'caption='[[1lnq]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1lnq]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. The February 2003 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Potassium Channels''  by Shuchismita Dutta and David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2003_2 10.2210/rcsb_pdb/mom_2003_2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LNQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LNQ FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lnq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lnq OCA], [https://pdbe.org/1lnq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lnq RCSB], [https://www.ebi.ac.uk/pdbsum/1lnq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lnq ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/MTHK_METTH MTHK_METTH] Calcium-gated potassium channel.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ln/1lnq_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lnq ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-LNQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 1LNQ with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb38_1.html Potassium Channels]]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LNQ OCA].
+*[[Potassium channel 3D structures|Potassium channel 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-Crystal structure and mechanism of a calcium-gated potassium channel., Jiang Y, Lee A, Chen J, Cadene M, Chait BT, MacKinnon R, Nature. 2002 May 30;417(6888):515-22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12037559 12037559]
+[[Category: Large Structures]]
 [[Category: Methanothermobacter thermautotrophicus]]
 [[Category: Potassium Channels]]
-[[Category: Single protein]]
+[[Category: RCSB PDB Molecule of the Month]]
-[[Category: Cadene, M.]]
+[[Category: Cadene M]]
-[[Category: Chait, B.T.]]
+[[Category: Chait BT]]
-[[Category: Chen, J.]]
+[[Category: Chen J]]
-[[Category: Jiang, Y.]]
+[[Category: Jiang Y]]
-[[Category: Lee, A.]]
+[[Category: Lee A]]
-[[Category: Mackinnon, R.]]
+[[Category: Mackinnon R]]
-[[Category: CA]]
-[[Category: helix bundle]]
-[[Category: membrane protein]]
-[[Category: rossman fold]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:03:20 2007''