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==LEUKOCIDIN F (HLGB) FROM STAPHYLOCOCCUS AUREUS==
==LEUKOCIDIN F (HLGB) FROM STAPHYLOCOCCUS AUREUS==
<StructureSection load='1lkf' size='340' side='right' caption='[[1lkf]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
<StructureSection load='1lkf' size='340' side='right'caption='[[1lkf]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1lkf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LKF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LKF FirstGlance]. <br>
<table><tr><td colspan='2'>[[1lkf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LKF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LKF FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LUKF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lkf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lkf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1lkf RCSB], [http://www.ebi.ac.uk/pdbsum/1lkf PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lkf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lkf OCA], [https://pdbe.org/1lkf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lkf RCSB], [https://www.ebi.ac.uk/pdbsum/1lkf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lkf ProSAT]</span></td></tr>
<table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/HLGB_STAAU HLGB_STAAU] Toxin that seems to act by forming pores in the membrane of the cell. Has a hemolytic and a leucotoxic activity.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
Check<jmol>
   <jmolCheckbox>
   <jmolCheckbox>
     <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lk/1lkf_consurf.spt"</scriptWhenChecked>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lk/1lkf_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
   </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lkf ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Staphylococcal LukF, LukS, HgammaII, and alpha-hemolysin are self-assembling, channel-forming proteins related in sequence and function. In the alpha-hemolysin heptamer, the channel-forming beta-strands and the amino latch make long excursions from the protomer core. Here we report the crystal structure of the water soluble form of LukF. In the LukF structure the channel-forming region folds into an amphipathic, three-strand beta-sheet and the amino latch forms a beta-strand extending a central beta-sheet. The LukF structure illustrates how a channel-forming toxin masks protein-protein and protein-membrane interfaces prior to cell binding and assembly, and together with the alpha-hemolysin heptamer structure, they define the end points on the pathway of toxin assembly.
Crystal structure of staphylococcal LukF delineates conformational changes accompanying formation of a transmembrane channel.,Olson R, Nariya H, Yokota K, Kamio Y, Gouaux E Nat Struct Biol. 1999 Feb;6(2):134-40. PMID:10048924<ref>PMID:10048924</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>


==See Also==
==See Also==
*[[Pore forming toxin%2C α-hemolysin|Pore forming toxin%2C α-hemolysin]]
*[[Pore forming toxin%2C α-hemolysin|Pore forming toxin%2C α-hemolysin]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
[[Category: Gouaux, J E.]]
[[Category: Gouaux JE]]
[[Category: Kamio, Y.]]
[[Category: Kamio Y]]
[[Category: Nariya, H.]]
[[Category: Nariya H]]
[[Category: Olson, R.]]
[[Category: Olson R]]
[[Category: Yokota, K.]]
[[Category: Yokota K]]
[[Category: Hemolysin]]
[[Category: Leukotoxin]]
[[Category: Pore-forming toxin]]
[[Category: Toxin]]

Latest revision as of 10:33, 14 February 2024

LEUKOCIDIN F (HLGB) FROM STAPHYLOCOCCUS AUREUSLEUKOCIDIN F (HLGB) FROM STAPHYLOCOCCUS AUREUS

Structural highlights

1lkf is a 1 chain structure with sequence from Staphylococcus aureus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.9Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

HLGB_STAAU Toxin that seems to act by forming pores in the membrane of the cell. Has a hemolytic and a leucotoxic activity.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1lkf, resolution 1.90Å

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OCA
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