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[[Image:1lj2.gif|left|200px]]


{{Structure
==Recognition of eIF4G by Rotavirus NSP3 reveals a basis for mRNA circularization==
|PDB= 1lj2 |SIZE=350|CAPTION= <scene name='initialview01'>1lj2</scene>, resolution 2.38&Aring;
<StructureSection load='1lj2' size='340' side='right'caption='[[1lj2]], [[Resolution|resolution]] 2.38&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=AU:GOLD+ION'>AU</scene>
<table><tr><td colspan='2'>[[1lj2]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Simian_rotavirus_A/SA11 Simian rotavirus A/SA11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LJ2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LJ2 FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.38&#8491;</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AU:GOLD+ION'>AU</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lj2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lj2 OCA], [https://pdbe.org/1lj2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lj2 RCSB], [https://www.ebi.ac.uk/pdbsum/1lj2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lj2 ProSAT]</span></td></tr>
|RELATEDENTRY=
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lj2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lj2 OCA], [http://www.ebi.ac.uk/pdbsum/1lj2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1lj2 RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/NSP3_ROTS1 NSP3_ROTS1] Involved in the shutoff of host protein synthesis. It is unclear whether it is required for the translation of viral mRNAs as well. Functions similarly to, and competes with the cellular poly(A)-binding protein PABPC1. Binds the conserved sequence 'GACC' at the 3' end of viral mRNAs and allows circularization of viral mRNAs in translation. Interacts with ZC3H7B/RoXaN and with the eukaryotic translation initiation factor eIF4G, using the same region employed by PABP-C1. NSP3 thus displaces PABPC1 from eIF4G, inhibiting the translation of cellular poly(A) mRNAs. Also responsible for the nuclear relocalization of PABPC1 upon rotavirus infection (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lj/1lj2_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lj2 ConSurf].
<div style="clear:both"></div>


'''Recognition of eIF4G by Rotavirus NSP3 reveals a basis for mRNA circularization'''
==See Also==
 
*[[Nonstructural protein 3D structures|Nonstructural protein 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
Rotaviruses, segmented double-stranded RNA viruses, co-opt the eukaryotic translation machinery with the aid of nonstructural protein 3 (NSP3), a rotaviral functional homolog of the cellular poly(A) binding protein (PABP). NSP3 binds to viral mRNA 3' consensus sequences and circularizes mRNA via interactions with eIF4G. Here, we present the X-ray structure of the C-terminal domain of NSP3 (NSP3-C) recognizing a fragment of eIF4GI. Homodimerization of NSP3-C yields a symmetric, elongated, largely alpha-helical structure with two hydrophobic eIF4G binding pockets at the dimer interface. Site-directed mutagenesis and isothermal titration calorimetry documented that NSP3 and PABP use analogous eIF4G recognition strategies, despite marked differences in tertiary structure.
[[Category: Homo sapiens]]
 
[[Category: Large Structures]]
==About this Structure==
[[Category: Simian rotavirus A/SA11]]
1LJ2 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Simian_rotavirus_a/sa11 Simian rotavirus a/sa11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LJ2 OCA].
[[Category: Burley SK]]
 
[[Category: Groft CM]]
==Reference==
Recognition of eIF4G by rotavirus NSP3 reveals a basis for mRNA circularization., Groft CM, Burley SK, Mol Cell. 2002 Jun;9(6):1273-83. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12086624 12086624]
[[Category: Protein complex]]
[[Category: Simian rotavirus a/sa11]]
[[Category: Burley, S K.]]
[[Category: Groft, C M.]]
[[Category: closed loop]]
[[Category: coiled coil]]
[[Category: eif4g]]
[[Category: homodimer]]
[[Category: mrna]]
[[Category: nsp3]]
[[Category: rotavirus]]
[[Category: translation]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:03:23 2008''

Latest revision as of 10:33, 14 February 2024

Recognition of eIF4G by Rotavirus NSP3 reveals a basis for mRNA circularizationRecognition of eIF4G by Rotavirus NSP3 reveals a basis for mRNA circularization

Structural highlights

1lj2 is a 4 chain structure with sequence from Homo sapiens and Simian rotavirus A/SA11. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.38Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NSP3_ROTS1 Involved in the shutoff of host protein synthesis. It is unclear whether it is required for the translation of viral mRNAs as well. Functions similarly to, and competes with the cellular poly(A)-binding protein PABPC1. Binds the conserved sequence 'GACC' at the 3' end of viral mRNAs and allows circularization of viral mRNAs in translation. Interacts with ZC3H7B/RoXaN and with the eukaryotic translation initiation factor eIF4G, using the same region employed by PABP-C1. NSP3 thus displaces PABPC1 from eIF4G, inhibiting the translation of cellular poly(A) mRNAs. Also responsible for the nuclear relocalization of PABPC1 upon rotavirus infection (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1lj2, resolution 2.38Å

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