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New page: left|200px<br /> <applet load="1kln" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kln, resolution 3.200Å" /> '''DNA POLYMERASE I K...
 
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[[Image:1kln.gif|left|200px]]<br />
<applet load="1kln" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1kln, resolution 3.200&Aring;" />
'''DNA POLYMERASE I KLENOW FRAGMENT (E.C.2.7.7.7) MUTANT/DNA COMPLEX'''<br />


==Overview==
==DNA POLYMERASE I KLENOW FRAGMENT (E.C.2.7.7.7) MUTANT/DNA COMPLEX==
Klenow fragment of Escherichia coli DNA polymerase I, which was, cocrystallized with duplex DNA, positioned 11 base pairs of DNA in a, groove that lies at right angles to the cleft that contains the polymerase, active site and is adjacent to the 3' to 5' exonuclease domain. When the, fragment bound DNA, a region previously referred to as the "disordered, domain" became more ordered and moved along with two helices toward the 3', to 5' exonuclease domain to form the binding groove. A single-stranded, 3', extension of three nucleotides bound to the 3' to 5' exonuclease active, site. Although this cocrystal structure appears to be an editing complex, it suggests that the primer strand approaches the catalytic site of the, polymerase from the direction of the 3' to 5' exonuclease domain and that, the duplex DNA product may bend to enter the cleft that contains the, polymerase catalytic site.
<StructureSection load='1kln' size='340' side='right'caption='[[1kln]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1kln]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. The March 2000 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''DNA Polymerase''  by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2000_3 10.2210/rcsb_pdb/mom_2000_3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KLN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KLN FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kln FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kln OCA], [https://pdbe.org/1kln PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kln RCSB], [https://www.ebi.ac.uk/pdbsum/1kln PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kln ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DPO1_ECOLI DPO1_ECOLI] In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity. It is able to utilize nicked circular duplex DNA as a template and can unwind the parental DNA strand from its template.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kl/1kln_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kln ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1KLN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 1KLN with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb3_1.html DNA Polymerase]]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KLN OCA].
*[[DNA polymerase 3D structures|DNA polymerase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Structure of DNA polymerase I Klenow fragment bound to duplex DNA., Beese LS, Derbyshire V, Steitz TA, Science. 1993 Apr 16;260(5106):352-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8469987 8469987]
[[Category: DNA Polymerase]]
[[Category: DNA Polymerase]]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Beese, L.S.]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Derbyshire, V.]]
[[Category: Beese LS]]
[[Category: T.A., Steitz..]]
[[Category: Derbyshire V]]
[[Category: ZN]]
[[Category: Steitz TA]]
[[Category: double helix]]
[[Category: protein-dna complex]]
 
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