1khr: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
← Older edit
No edit summary
No edit summary
 
(9 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1khr.gif|left|200px]]


<!--
==Crystal Structure of Vat(D) in Complex with Virginiamycin and Coenzyme A==
The line below this paragraph, containing "STRUCTURE_1khr", creates the "Structure Box" on the page.
<StructureSection load='1khr' size='340' side='right'caption='[[1khr]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1khr]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecium Enterococcus faecium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KHR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KHR FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
-->
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=VIR:VIRGINIAMYCIN+M1'>VIR</scene></td></tr>
{{STRUCTURE_1khr|  PDB=1khr  |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1khr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1khr OCA], [https://pdbe.org/1khr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1khr RCSB], [https://www.ebi.ac.uk/pdbsum/1khr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1khr ProSAT]</span></td></tr>
 
</table>
'''Crystal Structure of Vat(D) in Complex with Virginiamycin and Coenzyme A'''
== Function ==
 
[https://www.uniprot.org/uniprot/VATD_ENTFC VATD_ENTFC] Inactivates the A compounds of streptogramin antibiotics by acetylation, thus providing resistance to these antibiotics.
 
== Evolutionary Conservation ==
==Overview==
[[Image:Consurf_key_small.gif|200px|right]]
The streptogramin class of antibiotics act to inhibit bacterial protein synthesis, and their semisynthetic derivatives, such as dalfopristin-quinupristin (Synercid), are used to treat serious or life-threatening infections due to multiply antibiotic resistant bacteria. Acquired resistance of the nosocomial pathogen Enterococcus faecium to the group A component of natural and semisynthetic streptogramin mixtures is a prerequisite for the streptogramin resistance phenotype and is mediated by a streptogramin acetyltransferase. The crystal structure of Vat(D), a streptogramin acetyltransferase from a human urinary isolate of E. faecium, has been determined as an apoenzyme and in complex with either acetyl-CoA or virginiamycin M1 and CoA. These structures illustrate the location and arrangement of residues at the active site, and point to His 82 as a residue that may function as a general base. The structural similarity of Vat(D) to the xenobiotic acetyltransferase from Pseudomonas aeruginosa indicates similarities in the catalytic mechanism for these enzymes as well as several shared and distinctive antibiotic binding interactions between these enzymes and their respective substrates. These results reveal the molecular basis for a reaction by which Gram-positive cocci acquire resistance to a last resort antibiotic.
Check<jmol>
 
  <jmolCheckbox>
==About this Structure==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kh/1khr_consurf.spt"</scriptWhenChecked>
1KHR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecium Enterococcus faecium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KHR OCA].  
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==Reference==
  </jmolCheckbox>
Crystal structure of Vat(D): an acetyltransferase that inactivates streptogramin group A antibiotics., Sugantino M, Roderick SL, Biochemistry. 2002 Feb 19;41(7):2209-16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11841212 11841212]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1khr ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Enterococcus faecium]]
[[Category: Enterococcus faecium]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Roderick, S L.]]
[[Category: Roderick SL]]
[[Category: Sugantino, M.]]
[[Category: Sugantino M]]
[[Category: Acyltransferase]]
[[Category: Antibiotic resistance]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 22:45:50 2008''

Latest revision as of 10:25, 14 February 2024

Crystal Structure of Vat(D) in Complex with Virginiamycin and Coenzyme ACrystal Structure of Vat(D) in Complex with Virginiamycin and Coenzyme A

Structural highlights

1khr is a 6 chain structure with sequence from Enterococcus faecium. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.8Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

VATD_ENTFC Inactivates the A compounds of streptogramin antibiotics by acetylation, thus providing resistance to these antibiotics.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1khr, resolution 2.80Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=1khr&oldid=4050124"