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==2.9 Angstrom Resolution Cryo-EM 3-D Reconstruction of Close-packed PCV2 Virus-like Particles==
==2.9 Angstrom Resolution Cryo-EM 3-D Reconstruction of Close-packed PCV2 Virus-like Particles==
<StructureSection load='3jci' size='340' side='right'caption='[[3jci]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
<SX load='3jci' size='340' side='right' viewer='molstar' caption='[[3jci]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3jci]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Porcine_circovirus-2 Porcine circovirus-2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JCI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JCI FirstGlance]. <br>
<table><tr><td colspan='2'>[[3jci]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Porcine_circovirus_2 Porcine circovirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JCI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JCI FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3jci FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jci OCA], [http://pdbe.org/3jci PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3jci RCSB], [http://www.ebi.ac.uk/pdbsum/3jci PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3jci ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3jci FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jci OCA], [https://pdbe.org/3jci PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3jci RCSB], [https://www.ebi.ac.uk/pdbsum/3jci PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3jci ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/Q8B980_PCV2 Q8B980_PCV2]
Single-particle cryoelectron microscopy typically discards close-packed particle images as unusable data. Here, we report an image processing strategy and case study of obtaining near-atomic resolution 3D reconstructions from close-packed particles. Multiple independent de novo initial models were constructed to determine and cross-validate the particle parameters. The particles with consistent views were further refined including not only Euler angles and center positions but also defocus, astigmatism, beam tilt, and overall and anisotropic magnification. We demonstrated this strategy with a 2.9 A resolution reconstruction of a 1.67 MDa virus-like particle of a circovirus, PCV2, recorded on 86 photographic films. The map resolution was further validated with a phase-randomization test and local resolution assessment, and the atomic model was validated with MolProbity and EMRinger. Close-packed virus particles were thus shown not only to be useful for high-resolution 3D reconstructions but also to allow data collection at significantly improved throughput for near-atomic resolution reconstructions.


2.9 A Resolution Cryo-EM 3D Reconstruction of Close-Packed Virus Particles.,Liu Z, Guo F, Wang F, Li TC, Jiang W Structure. 2016 Jan 13. pii: S0969-2126(15)00511-0. doi:, 10.1016/j.str.2015.12.006. PMID:26777413<ref>PMID:26777413</ref>
==See Also==
 
*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3jci" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</SX>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Porcine circovirus-2]]
[[Category: Porcine circovirus 2]]
[[Category: Guo, F]]
[[Category: Guo F]]
[[Category: Jiang, W]]
[[Category: Jiang W]]
[[Category: Li, T C]]
[[Category: Li TC]]
[[Category: Liu, Z]]
[[Category: Liu Z]]
[[Category: Wang, F]]
[[Category: Wang F]]
[[Category: Consensus criterion]]
[[Category: Cross-validation]]
[[Category: De novo initial model]]
[[Category: Gold-standard fsc]]
[[Category: True fsc]]
[[Category: Virus like particle]]

Latest revision as of 11:43, 7 February 2024

2.9 Angstrom Resolution Cryo-EM 3-D Reconstruction of Close-packed PCV2 Virus-like Particles2.9 Angstrom Resolution Cryo-EM 3-D Reconstruction of Close-packed PCV2 Virus-like Particles

3jci, resolution 2.90Å

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