3jca: Difference between revisions

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<SX load='3jca' size='340' side='right' viewer='molstar' caption='[[3jca]], [[Resolution|resolution]] 4.80&Aring;' scene=''>
<SX load='3jca' size='340' side='right' viewer='molstar' caption='[[3jca]], [[Resolution|resolution]] 4.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3jca]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Mmtv Mmtv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JCA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JCA FirstGlance]. <br>
<table><tr><td colspan='2'>[[3jca]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Mouse_mammary_tumor_virus Mouse mammary tumor virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JCA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JCA FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3jca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jca OCA], [https://pdbe.org/3jca PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3jca RCSB], [https://www.ebi.ac.uk/pdbsum/3jca PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3jca ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3jca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jca OCA], [https://pdbe.org/3jca PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3jca RCSB], [https://www.ebi.ac.uk/pdbsum/3jca PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3jca ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/POL_MMTVB POL_MMTVB] During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase.
Retroviral integrase catalyses the integration of viral DNA into host target DNA, which is an essential step in the life cycle of all retroviruses. Previous structural characterization of integrase-viral DNA complexes, or intasomes, from the spumavirus prototype foamy virus revealed a functional integrase tetramer, and it is generally believed that intasomes derived from other retroviral genera use tetrameric integrase. However, the intasomes of orthoretroviruses, which include all known pathogenic species, have not been characterized structurally. Here, using single-particle cryo-electron microscopy and X-ray crystallography, we determine an unexpected octameric integrase architecture for the intasome of the betaretrovirus mouse mammary tumour virus. The structure is composed of two core integrase dimers, which interact with the viral DNA ends and structurally mimic the integrase tetramer of prototype foamy virus, and two flanking integrase dimers that engage the core structure via their integrase carboxy-terminal domains. Contrary to the belief that tetrameric integrase components are sufficient to catalyse integration, the flanking integrase dimers were necessary for mouse mammary tumour virus integrase activity. The integrase octamer solves a conundrum for betaretroviruses as well as alpharetroviruses by providing critical carboxy-terminal domains to the intasome core that cannot be provided in cis because of evolutionarily restrictive catalytic core domain-carboxy-terminal domain linker regions. The octameric architecture of the intasome of mouse mammary tumour virus provides new insight into the structural basis of retroviral DNA integration.
 
Cryo-EM reveals a novel octameric integrase structure for betaretroviral intasome function.,Ballandras-Colas A, Brown M, Cook NJ, Dewdney TG, Demeler B, Cherepanov P, Lyumkis D, Engelman AN Nature. 2016 Feb 18;530(7590):358-61. doi: 10.1038/nature16955. PMID:26887496<ref>PMID:26887496</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3jca" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</SX>
</SX>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Mmtv]]
[[Category: Mouse mammary tumor virus]]
[[Category: Ballandras-Colas, A]]
[[Category: Ballandras-Colas A]]
[[Category: Brown, M]]
[[Category: Brown M]]
[[Category: Cherepanov, P]]
[[Category: Cherepanov P]]
[[Category: Cook, N J]]
[[Category: Cook NJ]]
[[Category: Demeler, B]]
[[Category: Demeler B]]
[[Category: Dewdney, T G]]
[[Category: Dewdney TG]]
[[Category: Engelman, A N]]
[[Category: Engelman AN]]
[[Category: Lyumkis, D L]]
[[Category: Lyumkis DL]]
[[Category: Intasome]]
[[Category: Integrase]]
[[Category: Integration]]
[[Category: Retrovirus]]
[[Category: Viral protein]]

Latest revision as of 11:43, 7 February 2024

Core model of the Mouse Mammary Tumor Virus intasomeCore model of the Mouse Mammary Tumor Virus intasome

3jca, resolution 4.80Å

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