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==Insight into Three-dimensional structure of Maize Chlorotic Mottle Virus Revealed by Single Particle Analysis==
==Insight into Three-dimensional structure of Maize Chlorotic Mottle Virus Revealed by Single Particle Analysis==
<StructureSection load='3jb8' size='340' side='right' caption='[[3jb8]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
<SX load='3jb8' size='340' side='right' viewer='molstar' caption='[[3jb8]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3jb8]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Maize_chlorotic_mottle_virus Maize chlorotic mottle virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JB8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JB8 FirstGlance]. <br>
<table><tr><td colspan='2'>[[3jb8]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Maize_chlorotic_mottle_virus Maize chlorotic mottle virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JB8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JB8 FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3jb8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jb8 OCA], [http://pdbe.org/3jb8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3jb8 RCSB], [http://www.ebi.ac.uk/pdbsum/3jb8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3jb8 ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.6&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3jb8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jb8 OCA], [https://pdbe.org/3jb8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3jb8 RCSB], [https://www.ebi.ac.uk/pdbsum/3jb8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3jb8 ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/I6QQC4_MCMV I6QQC4_MCMV]
Maize chlorotic mottle virus (MCMV) is the only member of the Machlomovirus genus in the family Tombusviridae. Here, we obtained the Cryo-EM structure of MCMV by single particle analysis with most local resolution at approximately 4 A. The Calpha backbone was built based on residues with bulky side chains. The resolved C-terminus of the capsid protein subunit and obvious openings at the 2-fold axis demonstrated the compactness of the asymmetric unit, which indicates an important role in the stability of MCMV. The Asp116 residue from each subunit around the 5-fold and 3-fold axes contributed to the negative charges in the centers of the pentamers and hexamers, which might serve as a solid barrier against the leakage of genomic RNA. Finally, the loops most exposed on the surface were analyzed and are proposed to be potential functional sites related to MCMV transmission.


Insight into the three-dimensional structure of maize chlorotic mottle virus revealed by Cryo-EM single particle analysis.,Wang CY, Zhang QF, Gao YZ, Zhou XP, Ji G, Huang XJ, Hong J, Zhang CX Virology. 2015 Nov;485:171-8. doi: 10.1016/j.virol.2015.07.014. Epub 2015 Aug 11. PMID:26275511<ref>PMID:26275511</ref>
==See Also==
 
*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3jb8" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</SX>
[[Category: Large Structures]]
[[Category: Maize chlorotic mottle virus]]
[[Category: Maize chlorotic mottle virus]]
[[Category: Gao, Y Z]]
[[Category: Gao YZ]]
[[Category: Hong, J]]
[[Category: Hong J]]
[[Category: Huang, X J]]
[[Category: Huang XJ]]
[[Category: Ji, G]]
[[Category: Ji G]]
[[Category: Wang, C Y]]
[[Category: Wang CY]]
[[Category: Zhang, C X]]
[[Category: Zhang CX]]
[[Category: Zhang, Q F]]
[[Category: Zhang QF]]
[[Category: Zhou, X P]]
[[Category: Zhou XP]]
[[Category: Icosahedral virus]]
[[Category: Mcmv]]
[[Category: Single particle analysis]]
[[Category: Three-dimensional structure]]
[[Category: Transmission]]
[[Category: Virus]]

Latest revision as of 11:42, 7 February 2024

Insight into Three-dimensional structure of Maize Chlorotic Mottle Virus Revealed by Single Particle AnalysisInsight into Three-dimensional structure of Maize Chlorotic Mottle Virus Revealed by Single Particle Analysis

3jb8, resolution 3.60Å

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OCA
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