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| ==Atomic structure of a non-enveloped virus reveals pH sensors for a coordinated process of cell entry== | | ==Atomic structure of a non-enveloped virus reveals pH sensors for a coordinated process of cell entry== |
| <StructureSection load='3j9d' size='340' side='right' caption='[[3j9d]], [[Resolution|resolution]] 3.50Å' scene=''> | | <SX load='3j9d' size='340' side='right' viewer='molstar' caption='[[3j9d]], [[Resolution|resolution]] 3.50Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[3j9d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bluetongue_virus_1 Bluetongue virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J9D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J9D FirstGlance]. <br> | | <table><tr><td colspan='2'>[[3j9d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bluetongue_virus_1 Bluetongue virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J9D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J9D FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.5Å</td></tr> |
| <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3j9e|3j9e]]</td></tr> | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j9d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j9d OCA], [http://pdbe.org/3j9d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j9d RCSB], [http://www.ebi.ac.uk/pdbsum/3j9d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j9d ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j9d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j9d OCA], [https://pdbe.org/3j9d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j9d RCSB], [https://www.ebi.ac.uk/pdbsum/3j9d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j9d ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| | == Function == |
| == Publication Abstract from PubMed == | | [https://www.uniprot.org/uniprot/B7U676_9VIRU B7U676_9VIRU] |
| Viruses sense environmental cues such as pH to engage in membrane interactions for cell entry during infection, but how nonenveloped viruses sense pH is largely undefined. Here, we report both high- and low-pH structures of bluetongue virus (BTV), which enters cells via a two-stage endosomal process. The receptor-binding protein VP2 possesses a zinc finger that may function to maintain VP2 in a metastable state and a conserved His866, which senses early-endosomal pH. The membrane-penetration protein VP5 has three domains: dagger, unfurling and anchoring. Notably, the beta-meander motif of the anchoring domain contains a histidine cluster that can sense late-endosomal pH and also possesses four putative membrane-interaction elements. Exposing BTV to low pH detaches VP2 and dramatically refolds the dagger and unfurling domains of VP5. Our biochemical and structure-guided-mutagenesis studies support these coordinated pH-sensing mechanisms.
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| Atomic model of a nonenveloped virus reveals pH sensors for a coordinated process of cell entry.,Zhang X, Patel A, Celma CC, Yu X, Roy P, Zhou ZH Nat Struct Mol Biol. 2015 Dec 7. doi: 10.1038/nsmb.3134. PMID:26641711<ref>PMID:26641711</ref>
| | ==See Also== |
| | | *[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]] |
| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
| |
| <div class="pdbe-citations 3j9d" style="background-color:#fffaf0;"></div>
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| == References == | |
| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </SX> |
| [[Category: Bluetongue virus 1]] | | [[Category: Bluetongue virus 1]] |
| [[Category: Celma, C]] | | [[Category: Large Structures]] |
| [[Category: Patel, A]] | | [[Category: Celma C]] |
| [[Category: Roy, P]] | | [[Category: Patel A]] |
| [[Category: Zhang, X]] | | [[Category: Roy P]] |
| [[Category: Zhou, Z H]] | | [[Category: Zhang X]] |
| [[Category: Cell entry]]
| | [[Category: Zhou ZH]] |
| [[Category: Non-enveloped virus]]
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| [[Category: Ph sensor]]
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| [[Category: Viral protein]]
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