301d: Difference between revisions

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-[[Image:301d.jpg|left|200px]]
-<!--
+==CAPTURING THE STRUCTURE OF A CATALYTIC RNA INTERMEDIATE: RNA HAMMERHEAD RIBOZYME, MG(II)-SOAKED==
-The line below this paragraph, containing "STRUCTURE_301d", creates the "Structure Box" on the page.
+<StructureSection load='301d' size='340' side='right'caption='[[301d]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[301d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=301D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=301D FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-{{STRUCTURE_301d|  PDB=301d  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=301d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=301d OCA], [https://pdbe.org/301d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=301d RCSB], [https://www.ebi.ac.uk/pdbsum/301d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=301d ProSAT]</span></td></tr>
+</table>
-'''CAPTURING THE STRUCTURE OF A CATALYTIC RNA INTERMEDIATE: RNA HAMMERHEAD RIBOZYME, MG(II)-SOAKED'''
+==See Also==
+*[[Ribozyme 3D structures|Ribozyme 3D structures]]
+__TOC__
-==Overview==
+</StructureSection>
-The crystal structure of an unmodified hammerhead RNA in the absence of divalent metal ions has been solved, and it was shown that this ribozyme can cleave itself in the crystal when divalent metal ions are added. This biologically active RNA fold is the same as that found previously for two modified hammerhead ribozymes. Addition of divalent cations at low pH makes it possible to capture the uncleaved RNA in metal-bound form. A conformational intermediate, having an additional Mg(II) bound to the cleavage-site phosphate, was captured by freeze-trapping the RNA at an active pH prior to cleavage. The most significant conformational changes were limited to the active site of the ribozyme, and the changed conformation requires only small additional movements to reach a proposed transition-state.
+[[Category: Large Structures]]
+[[Category: Arnold JRP]]
-==About this Structure==
+[[Category: Klug A]]
-Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=301D OCA].
+[[Category: Murray JB]]
+[[Category: Scott WG]]
-==Reference==
+[[Category: Stoddard BL]]
-Capturing the structure of a catalytic RNA intermediate: the hammerhead ribozyme., Scott WG, Murray JB, Arnold JR, Stoddard BL, Klug A, Science. 1996 Dec 20;274(5295):2065-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8953035 8953035]
-[[Category: Arnold, J R.P.]]
-[[Category: Klug, A.]]
-[[Category: Murray, J B.]]
-[[Category: Scott, W G.]]
-[[Category: Stoddard, B L.]]
-[[Category: Catalytic rna]]
-[[Category: Loop]]
-[[Category: Rna hammerhead ribozyme]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 20:14:40 2008''