301d: Difference between revisions

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New page: left|200px<br /><applet load="301d" size="350" color="white" frame="true" align="right" spinBox="true" caption="301d, resolution 3.000Å" /> '''CAPTURING THE STRUC...
 
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'''CAPTURING THE STRUCTURE OF A CATALYTIC RNA INTERMEDIATE: RNA HAMMERHEAD RIBOZYME, MG(II)-SOAKED'''<br />


==Overview==
==CAPTURING THE STRUCTURE OF A CATALYTIC RNA INTERMEDIATE: RNA HAMMERHEAD RIBOZYME, MG(II)-SOAKED==
The crystal structure of an unmodified hammerhead RNA in the absence of, divalent metal ions has been solved, and it was shown that this ribozyme, can cleave itself in the crystal when divalent metal ions are added. This, biologically active RNA fold is the same as that found previously for two, modified hammerhead ribozymes. Addition of divalent cations at low pH, makes it possible to capture the uncleaved RNA in metal-bound form. A, conformational intermediate, having an additional Mg(II) bound to the, cleavage-site phosphate, was captured by freeze-trapping the RNA at an, active pH prior to cleavage. The most significant conformational changes, were limited to the active site of the ribozyme, and the changed, conformation requires only small additional movements to reach a proposed, transition-state.
<StructureSection load='301d' size='340' side='right'caption='[[301d]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[301d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=301D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=301D FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=301d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=301d OCA], [https://pdbe.org/301d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=301d RCSB], [https://www.ebi.ac.uk/pdbsum/301d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=301d ProSAT]</span></td></tr>
</table>


==About this Structure==
==See Also==
301D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=301D OCA].
*[[Ribozyme 3D structures|Ribozyme 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Capturing the structure of a catalytic RNA intermediate: the hammerhead ribozyme., Scott WG, Murray JB, Arnold JR, Stoddard BL, Klug A, Science. 1996 Dec 20;274(5295):2065-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8953035 8953035]
[[Category: Large Structures]]
[[Category: Protein complex]]
[[Category: Arnold JRP]]
[[Category: Arnold, J.R.P.]]
[[Category: Klug A]]
[[Category: Klug, A.]]
[[Category: Murray JB]]
[[Category: Murray, J.B.]]
[[Category: Scott WG]]
[[Category: Scott, W.G.]]
[[Category: Stoddard BL]]
[[Category: Stoddard, B.L.]]
[[Category: MG]]
[[Category: catalytic rna]]
[[Category: loop]]
[[Category: rna hammerhead ribozyme]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 21:34:23 2008''

Latest revision as of 11:38, 7 February 2024

CAPTURING THE STRUCTURE OF A CATALYTIC RNA INTERMEDIATE: RNA HAMMERHEAD RIBOZYME, MG(II)-SOAKEDCAPTURING THE STRUCTURE OF A CATALYTIC RNA INTERMEDIATE: RNA HAMMERHEAD RIBOZYME, MG(II)-SOAKED

Structural highlights

301d is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

See Also

301d, resolution 3.00Å

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