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[[Image:1kcf.gif|left|200px]]<br /><applet load="1kcf" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1kcf, resolution 2.30&Aring;" />
'''Crystal Structure of the Yeast Mitochondrial Holliday Junction Resolvase, Ydc2'''<br />


==Overview==
==Crystal Structure of the Yeast Mitochondrial Holliday Junction Resolvase, Ydc2==
Resolution of Holliday junctions into separate DNA duplexes requires enzymatic cleavage of an equivalent strand from each contributing duplex at or close to the point of strand exchange. Diverse Holliday junction-resolving enzymes have been identified in bacteria, bacteriophages, archaea and pox viruses, but the only eukaryotic examples identified so far are those from fungal mitochondria. We have now determined the crystal structure of Ydc2 (also known as SpCce1), a Holliday junction resolvase from the fission yeast Schizosaccharomyces pombe that is involved in the maintenance of mitochondrial DNA. This first structure of a eukaryotic Holliday junction resolvase confirms a distant evolutionary relationship to the bacterial RuvC family, but reveals structural features which are unique to the eukaryotic enzymes. Detailed analysis of the dimeric structure suggests mechanisms for junction isomerization and communication between the two active sites, and together with site-directed mutagenesis identifies residues involved in catalysis.
<StructureSection load='1kcf' size='340' side='right'caption='[[1kcf]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1kcf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KCF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KCF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kcf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kcf OCA], [https://pdbe.org/1kcf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kcf RCSB], [https://www.ebi.ac.uk/pdbsum/1kcf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kcf ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CCE1_SCHPO CCE1_SCHPO] Capable of resolving Holliday junctions. Specific for 4-way junctions. Seems to be important for the maintenance of mitochondrial DNA. Cleaves fixed junctions at the point of strand exchange. Cleaves after 5'-CT-3' and 5'-TT-3' sequences.<ref>PMID:9343409</ref> <ref>PMID:10954073</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kc/1kcf_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kcf ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1KCF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KCF OCA].
*[[Resolvase|Resolvase]]
 
*[[Resolvase 3D structures|Resolvase 3D structures]]
==Reference==
== References ==
Crystal structure of the fission yeast mitochondrial Holliday junction resolvase Ydc2., Ceschini S, Keeley A, McAlister MS, Oram M, Phelan J, Pearl LH, Tsaneva IR, Barrett TE, EMBO J. 2001 Dec 3;20(23):6601-11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11726496 11726496]
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Schizosaccharomyces pombe]]
[[Category: Schizosaccharomyces pombe]]
[[Category: Single protein]]
[[Category: Barrett TE]]
[[Category: Barrett, T E.]]
[[Category: Ceschini S]]
[[Category: Ceschini, S.]]
[[Category: Keeley A]]
[[Category: Keeley, A.]]
[[Category: McAlister MSB]]
[[Category: McAlister, M S.B.]]
[[Category: Oram M]]
[[Category: Oram, M.]]
[[Category: Pearl LH]]
[[Category: Pearl, L H.]]
[[Category: Phelan J]]
[[Category: Phelan, J.]]
[[Category: Tsaneva IR]]
[[Category: Tsaneva, I R.]]
[[Category: SO4]]
[[Category: beta-alpha-beta motif]]
[[Category: ruvc resolvase family]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:32:31 2008''

Latest revision as of 10:45, 7 February 2024

Crystal Structure of the Yeast Mitochondrial Holliday Junction Resolvase, Ydc2Crystal Structure of the Yeast Mitochondrial Holliday Junction Resolvase, Ydc2

Structural highlights

1kcf is a 2 chain structure with sequence from Schizosaccharomyces pombe. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.3Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CCE1_SCHPO Capable of resolving Holliday junctions. Specific for 4-way junctions. Seems to be important for the maintenance of mitochondrial DNA. Cleaves fixed junctions at the point of strand exchange. Cleaves after 5'-CT-3' and 5'-TT-3' sequences.[1] [2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ White MF, Lilley DM. Characterization of a Holliday junction-resolving enzyme from Schizosaccharomyces pombe. Mol Cell Biol. 1997 Nov;17(11):6465-71. PMID:9343409
  2. ↑ Doe CL, Osman F, Dixon J, Whitby MC. The Holliday junction resolvase SpCCE1 prevents mitochondrial DNA aggregation in Schizosaccharomyces pombe. Mol Gen Genet. 2000 Jul;263(6):889-97. PMID:10954073

1kcf, resolution 2.30Å

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