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[[Image:1k8t.gif|left|200px]]


{{Structure
==Crystal structure of the adenylyl cyclase domain of anthrax edema factor (EF)==
|PDB= 1k8t |SIZE=350|CAPTION= <scene name='initialview01'>1k8t</scene>, resolution 2.6&Aring;
<StructureSection load='1k8t' size='340' side='right'caption='[[1k8t]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=NI:NICKEL (II) ION'>NI</scene>
<table><tr><td colspan='2'>[[1k8t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. The April 2002 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Anthrax Toxin''  by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2002_4 10.2210/rcsb_pdb/mom_2002_4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K8T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K8T FirstGlance]. <br>
|ACTIVITY= [http://en.wikipedia.org/wiki/Adenylate_cyclase Adenylate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.1 4.6.1.1]  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
}}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k8t OCA], [https://pdbe.org/1k8t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k8t RCSB], [https://www.ebi.ac.uk/pdbsum/1k8t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k8t ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CYAA_BACAN CYAA_BACAN] One of the three proteins composing the anthrax toxin, the agent which infects many mammalian species and that may cause death. EF is a calmodulin-dependent adenylyl cyclase that, when associated with PA, causes edema. EF is not toxic by itself and it is required for the survival of germinated spores within macrophages at the early stages of infection. Provokes dramatic elevation of intracellular cAMP levels in the host.


'''Crystal structure of the adenylyl cyclase domain of anthrax edema factor (EF)'''
==See Also==
 
*[[Anthrax edema factor 3D structures|Anthrax edema factor 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
Oedema factor, a calmodulin-activated adenylyl cyclase, is important in the pathogenesis of anthrax. Here we report the X-ray structures of oedema factor with and without bound calmodulin. Oedema factor shares no significant structural homology with mammalian adenylyl cyclases or other proteins. In the active site, 3'-deoxy-ATP and a single metal ion are well positioned for catalysis with histidine 351 as the catalytic base. This mechanism differs from the mechanism of two-metal-ion catalysis proposed for mammalian adenylyl cyclases. Four discrete regions of oedema factor form a surface that recognizes an extended conformation of calmodulin, which is very different from the collapsed conformation observed in other structures of calmodulin bound to effector peptides. On calmodulin binding, an oedema factor helical domain of relative molecular mass 15,000 undergoes a 15 A translation and a 30 degrees rotation away from the oedema factor catalytic core, which stabilizes a disordered loop and leads to enzyme activation. These allosteric changes provide the first molecular details of how calmodulin modulates one of its targets.
 
==About this Structure==
1K8T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. The following page contains interesting information on the relation of 1K8T with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb28_1.html Anthrax Toxin]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K8T OCA].
 
==Reference==
Structural basis for the activation of anthrax adenylyl cyclase exotoxin by calmodulin., Drum CL, Yan SZ, Bard J, Shen YQ, Lu D, Soelaiman S, Grabarek Z, Bohm A, Tang WJ, Nature. 2002 Jan 24;415(6870):396-402. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11807546 11807546]
[[Category: Adenylate cyclase]]
[[Category: Anthrax Toxin]]
[[Category: Anthrax Toxin]]
[[Category: Bacillus anthracis]]
[[Category: Bacillus anthracis]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Bard, J.]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Bohm, A.]]
[[Category: Bard J]]
[[Category: Drum, C L.]]
[[Category: Bohm A]]
[[Category: Grabarek, Z.]]
[[Category: Drum CL]]
[[Category: Lu, D.]]
[[Category: Grabarek Z]]
[[Category: Shen, Y Q.]]
[[Category: Lu D]]
[[Category: Soelaiman, S.]]
[[Category: Shen Y-Q]]
[[Category: Tang, W J.]]
[[Category: Soelaiman S]]
[[Category: Yan, S Z.]]
[[Category: Tang W-J]]
[[Category: NI]]
[[Category: Yan S-Z]]
[[Category: SO4]]
[[Category: adenylyl cyclase]]
[[Category: anthrax]]
[[Category: calmodulin]]
[[Category: edema factor]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:14:03 2008''

Latest revision as of 10:45, 7 February 2024

Crystal structure of the adenylyl cyclase domain of anthrax edema factor (EF)Crystal structure of the adenylyl cyclase domain of anthrax edema factor (EF)

Structural highlights

1k8t is a 1 chain structure with sequence from Bacillus anthracis. The April 2002 RCSB PDB Molecule of the Month feature on Anthrax Toxin by David S. Goodsell is 10.2210/rcsb_pdb/mom_2002_4. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.6Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CYAA_BACAN One of the three proteins composing the anthrax toxin, the agent which infects many mammalian species and that may cause death. EF is a calmodulin-dependent adenylyl cyclase that, when associated with PA, causes edema. EF is not toxic by itself and it is required for the survival of germinated spores within macrophages at the early stages of infection. Provokes dramatic elevation of intracellular cAMP levels in the host.

See Also

1k8t, resolution 2.60Å

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OCA
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